摘要
目的探讨藻酸钙凝胶、成骨细胞、骨粉复合构建的可塑形组织工程骨修补兔颅骨缺损后的形态学变化和成骨效果。方法28只日本大耳白兔,随机分为A(n=20)、B(n=8)两组,手术在兔颅顶骨矢状缝两侧分别各建立一个直径1cm的圆形全层缺损,用两种方法:藻酸钙凝胶、成骨细胞、骨粉和藻酸钙凝胶、骨粉分别构建组成可塑形的组织工程骨复合材料,分别填补修复A组兔颅骨左右两侧的缺损,B组为空白对照组,通过大体、组织学、X线观察材料的形态变化、成骨情况,并对X线片和组织学切片进行评分。结果材料植入后,局部未见红肿、积液、渗出等异常反应。①藻酸钙凝胶-成骨细胞-骨粉组:修补后12周颅骨缺损基本被硬性组织所修复,镜下见修复材料大多被骨组织替代,骨粉基本被吸收,有块状凝胶残留其中,组织学评分为(5.50±1.00)。X线片见兔颅骨缺损处有高密度骨痂影存在,布满整个缺损区,X线片评分为(3.25±0.95)。②藻酸钙凝胶-骨粉组:修补后12周部分颅骨缺损被硬性组织所修复,镜下见修复材料部分转变成骨组织,骨粉基本被吸收,有凝胶残留其中,组织学评分为(3.25±1.50)。X线片见兔颅骨缺损处有高密度骨痂影存在,主要分布在缺损区的边缘部位,X线片评分(2.25±0.25)。③空白对照组:骨缺损主要被膜样纤维组织修复,在紧邻骨缺损边缘处有硬?
Objective To investigate the morphological change and bone regeneration after the repair of rabbit skull defect with engineered bone prefabricated respectively with combination of alginate gel, osteoblast and bone granules, and with combination of alginate and bone granules. Methods 28 rabbits were randomized into Group A (n=20) and Group B(n=8). A skull defect 1cm in diameter was made on the right and the left sides of every rabbit respectively. The left and right skull defects in Group A were respectively repaired with alginate gel, osteoblast and bone granules as well as with alginate gel and bone granules. Group B was served as the empty control. Gross, histological and x-ray observations were done to evaluate the morphological change and bone regeneration. X ray and histological scorings were made. Results 12 weeks after the operation, the left skull defects in Group A were almost entirely repaired by hard tissue. The alginate gel, osteoblast and bone granule material changed into bone tissue, while only a little bone granules and some residuary alginate gel remained. The histological scoring was 5.5±1. In Group B the skull defects were repaired mainly by membrane-like soft tissue, while only a little bone tissue appeared in the marginal area. The histological scoring was 1.5±0.5. X ray plates showed that the area of left skull defect in Group A was full of high density shade while the high density shade occurred only in the marginal area of skull defect in the control group. Conclusions Engineered bone prefabricated with alginate gel, osteoblast and bone granule can be formed into different shapes of the bone defects and well capable of bone tissue regeneration. The problem of alginate gel remains is still to be solved.
出处
《中华创伤骨科杂志》
CAS
CSCD
2004年第7期756-759,766,共5页
Chinese Journal of Orthopaedic Trauma
基金
国家高科技研究开发计划资助项目(863)重大专项(2002AA205011)
国家高科技研究发展计划资助项目(863)(2001AA216011)
国家自然科学基金重点项目基金(39830100)
关键词
藻酸盐
凝胶
骨粉
成骨细胞
骨缺损
组织工程
Alginate acid
Bone granule
Osteoblast
Bone defect
Engineered bone
Tissue engineering
