摘要
以甜樱桃‘雷尼’为试材,利用桃基因组设计引物克隆获得两个ORF分别为519和516 bp的TFL1基因全长序列,分别编码172和171个氨基酸,均具有保守的PEBP结构域和9个底物结合位点,符合TFL1家族基因的典型特征,分别命名为Pa TFL1a和Pa TFL1b。对‘雷尼’×2121杂交组合3年生和4年生实生后代调查发现其始花节位为28.72±1.34,童程为(63.33±3.19)cm;实时荧光定量PCR分析表明其新梢顶端分生组织中,童区内Pa TFL1a和Pa TFL1b表达量远高于成年区,且成年区内的表达量随着节位增高而增加;对1~4年生实生后代不同枝龄的顶端分生组织中Pa TFL1a和Pa TFL1b表达量检测发现随树龄和枝龄的上升其表达量下调明显。因此,Pa TFL1a和Pa TFL1b基因与甜樱桃童期向成年阶段转变相关。在第30节位新梢成熟叶片中Pa TFL1a表达量不随树龄的改变而变化,而Pa TFL1b的表达与顶端分生组织中规律一致,可以作为甜樱桃‘雷尼’阶段转变过程的检测标记。
Rainier sweet cherry was used as test material. Using the peach genome to design primers,the full-length sequences of two genes whose ORF were 519 bp and 516 bp encoded 172 and 171 amino acids respectively. They had the conserved PEBP domain and 9 substrate binding sites,and were in line with the typical characteristics of TFL1 gene family,which named Pa TFL1 a and Pa TFL1 b respectively. A survey on 3,4-year old hybrid offspring of Rainier × 2121 was made. Its first flower node was 28.72 ± 1.34,the juvenile span was(63.33 ± 3.19)cm. The q RT-PCR analysis on the shoot apical meristem from 3,4-year old hybrid offspring of Rainier × 2121 showed that the relative expression quantity of Pa TFL1 a and Pa TFL1 b in juvenile zone was much higher than that in adult zone. The relative expression quantity of them were increased with the nodes increasing in adult zone. In shoot apical meristem from 1–4-year old hybrid offspring of Rainier × 2121,the relative expression quantity of two genes were reduced by the ages of trees and branches. Therefore,Pa TFL1 a and Pa TFL1 b genes may be associated with the sweet cherry stage transformation. In the leaves of 30 th node,the relative expression quantity of Pa TFL1 a did not change by age,while the relative expression quantity of Pa TFL1 b was consistent with apical meristems,and it can be used as a convenient detection marker for the stage transformation of Rainier.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第6期1031-1039,共9页
Acta Horticulturae Sinica
基金
‘十二五’科技支撑计划项目(2013BAD02B03)
国家自然科学基金项目(31272123)
国家自然科学基金青年基金项目(31401827)
科技部科技基础性工作专项(2012FY110100)
北京市科委重大项目(D131100000113001)
北京市农林科学院科技创新能力建设专项(KJCX20140110
KJCX201400202)
关键词
甜樱桃
TFL1家族基因
成花
阶段转变
sweet cherry
TFL1 family gene
flower formation
stage transformation
