摘要
以抗旱力弱的毛果绣线菊与抗旱力强的华北绣线菊为试验材料,采用不同程度干旱处理,在测定其光合作用变化的基础上,采用蛋白质组学技术研究两种绣线菊光合作用相关蛋白表达的变化。结果表明:干旱胁迫两种绣线菊光合能力降低,光补偿点和光饱和点降低,说明利用弱光能力提高,华北绣线菊适应弱光环境的能力强于毛果绣线菊。不同水平干旱处理分别鉴定出涉及能量代谢与运输的相关蛋白20种。干旱胁迫下毛果绣线菊中卡尔文循环途径相关酶、光系统Ⅱ中多数放氧复合蛋白和捕光色素复合蛋白、参与光合磷酸化的酶发生显著上调和下调表达;华北绣线菊中参与卡尔文循环代谢相关酶、PSⅠ中反应中心蛋白和参与光合电子传递的蛋白、PSⅡ中捕光色素复合蛋白、参与光合磷酸化与氧化磷酸化的蛋白发生明显的上调和下调表达。干旱胁迫使抗旱力不同的毛果绣线菊和华北绣线菊光合作用相关蛋白均受到影响,毛果绣线菊中多是能量代谢相关蛋白发生显著变化,华北绣线菊中多是能量运输相关蛋白发生显著变化。
Spiraea thichocarpa Nakai with poor drought resistance and S. fritschiana Schneid with strong drought resistance were used as experimental materials under different levels of drought treatments. Proteomics technology was used for studying the changes of expressions of proteins related to photosynthesis of two Spiraea species which was on the basic of measuring the changes of photosynthesis. The results show that the photosynthetic capacities of two Spiraea species decreased under drought stress,light compensation point(LCP)and light saturation point(LSP)decreased,but their capacity of using weak light increased,S. fritschiana Schneid with stronger drought resistance showed a better ability of adapting weak light than that of S. thichocarpa Nakai. Twenty kinds of proteins related to photosynthesis involved in energy metabolism and transport were identified separately under different levels of drought treatments. In S. thichocarpa Nakai,the expressions included the enzymes in Calvin Cycle and photophosphorylation,the most oxygen-evolving complex proteins and the chlorophyll a/b binding proteins of photosystem Ⅱshowed obvious up and down regulation under drought stress. While in S. fritschiana Schneid,the expressions included the enzymes in Calvin Cycle,the reaction center proteins and electron transport proteins of photosystemⅠ,the chlorophyll a/b binding proteins of photosystemⅡ,the proteins involved in photophosphorylation and oxidative photosphorylation also showed obvious up and down regulation under drought stress. The proteins related to photosynthesis of two Spiraea species were effected under drought stress. In S. thichocarpa Nakai,it was the proteins related to energy metabolism changed significantly. In S. fritschiana Schneid,it was the proteins related to energy transport changed significantly.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第5期917-929,共13页
Acta Horticulturae Sinica
基金
中国博士后基金面上项目(415152)
黑龙江省博士后基金项目(LBH-Z09288)
东北农业大学博士启动项目
关键词
绣线菊
干旱处理
光合作用
光合作用相关蛋白
光合能力
Spiraea
drought stress
photosynthesis
proteins related to photosynthesis
photosynthetic capacity
