摘要
根据番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)基因组序列的复制酶保守区设计1对引物JC1,对石家庄具有典型番茄黄化曲叶病毒症状的番茄样品进行检测,经PCR扩增得到626 bp的片段,序列分析比对表明其为TYLCV的一部分。根据上述序列设计1对特异引物QC,通过PCR分离石家庄的TYLCV全长序列并进行测序。同时利用已报道的DNA-B的通用引物CR01/CR02进行PCR扩增。结果表明石家庄分离物只含有DNA-A组分,全长为2 781 bp(Gen Bank登录号:KF612971),命名为TYLCV-Shijiazhuang。基因组序列比较发现,该分离物与TYLCV-Israel株系相似性为99.0%。基因组序列系统进化分析表明,石家庄病毒分离物与北京分离物TYLCV-Beijing3(Gen Bank登录号:GU983859)、山东分离物TYLCV-SDSG-XC(Gen Bank登录号:KC999851)序列相似性很高,均属于TYLCV-Israel分支。
A 626 bp fragment of replication protein region of TYLCV(Tomato yellow leaf curl virus)was cloned using PCR with primers JC1 from Shijiazhuang samples with typical symptoms of yellow leaf curl,and sequence analysis confirmed that it was a part of TYLCV. Then the full-length of TYLCV was obtained with a pair of primers QC and sequenced. The already reported universary primers CR01/CR02 for DNA-B were used for amplification and the results showed that only DNA-A containing 2 781 bp nucleotides and it was named as TYLCV-shijiazhuang(Gen Bank accession No. KF612971). Sequence analysis showed that TYLCV-shijiazhuang was similar to TYLCV-Israel for 99.0%. However,it was sometimes expceptional that TYLCV-Shijiazhuang isolate was most closely related to TYLCV-Beijing3(Gen Bank accession No. GU983859),TYLCV-SDSG-XC(Gen Bank accession No. KC999851),and they all belonged to the same monophyletic clusters of TYLCV-Israel isolate.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第1期167-173,共7页
Acta Horticulturae Sinica
基金
河北省财政专项计划项目(2013055003)
河北省农林科学院院士合作项目(A2012050601)
河北省科技支撑计划项目(14226310D)
