摘要
目的 建立人血浆中消癌平高效液相色谱(HPLC)测定法.方法 采用HPLC法,色谱柱为C18柱(250 mm×4.6 mm,5 km),流动相为乙腈-0.4%磷酸溶液(13:87),流速为1.0 ml/min,柱温为室温,检测波长327 nm.结果 绿原酸在浓度2.5～60.0 mg/L范围内呈良好的线性关系(r=0.9996),最低检测浓度为0.25 mg/L.方法 回收率为100.1%～102.6%,提取回收率为78.6%～80.3%.日内、日间相对标准偏差值(RSD)均小于3.8%.结论 反相高效液相色谱法快速、简便、准确,可用于人血浆中消癌平注射液中绿原酸含量测定.
Objective To develop a HPLC method for the determination of chlorogenic acid in xiaoaiping injection in human plasma.Methods The analytical column was packed with Hypersil ODS C18 (250 mm×4.6 nm,5 μm).The mobile phase was acetonitrile-0.4% phosphoric acid solution(13:87).The flow rate was 1 ml/min,and detection wave length was set at 327 nm.Results The linear ranges were 2.5～60.0 mg/L chlorogenic acid in human plasma,with the correlation coefficients of 0.9996.The Low limit of detection was 0.25 μg/ml.The method and extraction recovery were 100.1%～102.6%and 78.6%～80.3%.The relative standard deviation value for the within-day and between-day precision were less than 3.9%.Conclusion The method is simple,sensitive for the determination of chlorogenic acid in xiaoaiping injection in human plasma.
出处
《肿瘤研究与临床》
CAS
2008年第3期158-160,共3页
Cancer Research and Clinic
作者简介
Correspondingauthor:YANKe-li,Email:yankeli@tom.com;阎克里,男,学士,副主任药师.通讯作者:阎克里,Email:yankeli@tom.com;
