摘要
铜绿微囊藻和蛋白核小球藻是藻类水华常见的优势藻,二者形状大小较为相似,不易区分,采用浮游植物流式细胞仪,初步研究了铜绿微囊藻和蛋白核小球藻的快速检测和区分技术。通过对比选择具有区分优势的参数指标,即代表细胞内颗粒复杂程度的侧向散射信号(SWS)的均值指标和代表叶绿素含量的红色荧光信号(FL Red)的均值指标,对两藻种进行了多维度的筛选和区分。根据选择的参数指标,导出对应的特征信号图谱,进一步考察其信号图谱的特征性。经研究表明区分藻种的一级指标是信号强度,二级指标是信号光滑度。
Microcystis aeruginosa and Chlorella pyrenoidosa are very common in cyanobacterial,they are similar both in size and shape and is not easy to distinguish,use the phytoplankton flow cytometry instrument to make some preliminary studies on the rapid detection and distinguish of M.aeruginosa and C.pyrenoidosa.Though the comparement of the parameters index to select the two with distinguish advantage,SWS Average which represents the degree of complexity of intracellular grnules and FL Red Average which behalf of the chlorophyll content,to make the multidimensional screening and distinguish of the two algae species.According to the selected parameters index,export corresponding characteristic signal pattern,Further investigated the characteristic of its signal spectrum.The research shows that the distinction between two algae species can be made by two indicators,the first is signal intensity and the second is signal smoothness.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2013年第S1期282-285,共4页
Environmental Science & Technology
关键词
流式细胞仪
快速区分
铜绿微囊藻
蛋白核小球藻
flow cytometry
rapid detection
Microcystis aeruginosa
Chlorella pyrenoidosa
