摘要
目的观察密蒙花总黄酮对去势雄鼠干眼病大鼠模型基础泪液分泌量、泪膜稳定性和角膜组织中凋亡相关蛋白Fas、FasL表达的影响,探讨密蒙花总黄酮抗性激素水平失调导致的大鼠干眼病的作用机制。方法 1月龄健康雄性Wistar大鼠150只,采用随机数字表法分为5组,每组30只,分别为:A组:正常组;B组:假手术组;C组:手术对照组;D组:雄激素治疗组(肌肉注射丙酸睾酮);E组:密蒙花总黄酮治疗组(密蒙花总黄酮灌胃给药);将C组、D组、E组实验鼠切除双侧睾丸及附睾以制作干眼病模型;B组只切开阴囊而不切除睾丸,作为假手术组;A组不做任何处理。各组在造模后1周,待伤口基本愈合后开始用药。各组实验动物分别于用药1个月、3个月、5个月后随机取10只,行Schirmer I试验、测量泪膜破裂时间,然后处死各组动物,取双眼角膜组织用免疫组织化学的方法检测Fas、FasL蛋白的表达情况。结果用药后5个月,D组、E组Schirmer I试验测量值分别为(5.41±1.32)mm和(5.32±0.95)mm,均明显高于C组的(3.71±0.91)mm(均为P<0.05);泪膜破裂时间分别为(9.34±0.87)s和(9.03±0.98)s,均明显长于C组的(7.78±1.07)s(均为P<0.05)。用药5个月后角膜组织中Fas蛋白光密度值在D组、E组分别为0.388±0.042和0.400±0.045,均明显低于C组的0.618±0.067(均为P<0.05);角膜组织中FasL蛋白光密度值在D组、E组分别为0.379±0.015和0.417±0.028,均明显低于C组的0.680±0.038(均为P<0.05)。D组、E组间各指标差异均无统计学意义(均为P>0.05)。结论密蒙花总黄酮可减少角膜组织中Fas和FasL的表达,抑制细胞凋亡,产生同丙酸睾酮治疗干眼病相似的效果。角膜细胞凋亡是雄激素缺乏导致干眼病的机制之一,且凋亡相关蛋白Fas和FasL的表达增加是导致角膜细胞凋亡的原因之一。
Objective To observe the effects of total buddleja flavonoids on the basal tear secretion amount,tear film stability and expression of apoptosis related gene Fas,FasL in castrated male rat model with xeroma and discuss the mechanism of rat xeroma caused by total buddleja flavones' anti-sex hormones disorders. Methods A total 150 healthy Wistar male rats with 1 month old were randomly divided into normal group(group A),sham operation group(group B),surgery control group(group C),androgen treated group(group D)and buddleja flavonoidstreated group(group E),30 rats in each group.The rats in group C,D,E were excised the bilateral testicle and epididymis to establish the xeroma model, group B were only incised scrotal without removal of the testicles,group A did nothing.One week after modeling when the wound was to be healed,drugs were given to each group.Ten rats were randomly selected in each group at the 1 month,3 months and 5 months after given drugs,respectively,Schirmer I test and tear breakup time measurement were performed.After that,they were executed to take the corneal tissues,the expression of apoptosis related gene Fas,FasL were detected with immunohistochemistry method.Results For group D and E,Schirmer I test measurement value at 5 months after given drugs were(5.41±1.32)mm and(5.32±0.95)mm,respectively,which were significantly higher than(3.71 ±0.91)mm of group C(all P<0.05).For group D and E,tear film breakup time were(9.34±0.87)s and(9.03±0.98)s,respectively,Which were significantly longer than(7.78±1.07)s of group C(all P<0.05).For group D and E,the optical density of Fas expression in corneal tissues were 0.388±0.042 and 0.400±0.045,respectively,which were significantly lower than 0.618±0.067 of group C(all P<0.01).For group D and E,the optical density FasL expression in corneal tissues were 0.379±0.015 and 0.417±0.028,respectively,which were significantly lower than 0.680±0.038 of group C(all P<0.01).There was no statistical difference between group D and E(all P>0.05).Conclusion Total buddleja flavonoids can reduce the expression of Fas and FasL in corneal tissues and inhibit the apoptosis,which produces the similar treatment effect of xeroma with testosterone propionate.The apoptosis of corneal cell is one of the mechanisms for androgen deficiency leading to xeroma and increased expression of apoptosis related protein Fas and FasL is one of the reasons of corneal cell apoptosis.
出处
《眼科新进展》
CAS
北大核心
2013年第12期1110-1114,共5页
Recent Advances in Ophthalmology
基金
国家自然科学基金面上项目(编号:30772824)
教育部高等学校博士学科点专项科研基金(编号:200805410004)
湖南省自然科学基金(编号:07JJ3049)
湖南省科技厅科研基金(编号:2009FJ3001)
2008湖南省研究生创新基金(编号:2008第28号)
国家中医药管理局中医眼科学重点学科建设项目
湖南省中医五官科学重点学科建设项目~~
