摘要
目的探讨骨碎补总黄酮(Total Flavonoids of Rhizoma Drynariae,TFRD)对去卵巢骨质疏松(osteoporosis,OP)大鼠骨内谷氨酸信号的影响,以明确其通过参与大鼠骨内谷氨酸信号通路介导骨重建的可能机制。方法 45只3月龄SPF级雌性SD大鼠随机分为假手术组(Sham,n=15)和去卵巢组(OVX,n=30)。采取双侧卵巢切除术复制OP大鼠模型,14周后行双能X线测定骨密度,模型复制成功,将OVX组随机分为模白组(OVX,n=15)和给药组(OVX+TFRD,n=15)。药物干预12周后处死动物,免疫组化法观察左股骨骨骺端谷氨酸(Glutamate,Glu)、代谢型Glu受体-5(metabotropic glutamate receptor5,mGluR5)及L-谷氨酸/L-门冬氨酸转运体(Glutamate/Aspartate transporter,EAAT1)表达。结果 Glu和mGluR5主要分布于骨髓区细胞和紧贴骨髓腔壁的成骨细胞(osteoblasts,OB);Glu的阳性标记在Sham、OVX和OVX+TFRD组的表达差异均无统计学意义;OVX+TFRD组代谢型受体mGluR5的阳性表达显著高于Sham组(P=0.009),而Sham组与OVX组之间的差异无统计学意义;转运子EAAT1除了大量分布于骨髓区细胞以外,还有少量表达于骨陷窝中的骨细胞,其阳性表达在Sham、OVX和OVX+TFRD组的差异均无统计学意义。结论在骨内Glu信号通路中,TFRD可显著提高代谢型受体mGluR5的表达,但尚不能认为其对Glu和转运子EAAT1有影响。
Objective To observe the expression of Glu、mGluR 5 and EAAT 1 in bone tissues of ovariectomized osteoporotic rats and the effects of Total Flavonoids of Rhizoma Drynariae (TFRD) on it. Methods 45 SPF 3-month-old Sprague-Dawley (SD) female rats were randomly divided into sham operation (Sham, n=15) group and ovariectomized (OVX, n=30) group. The osteoporotic(OP) model was established by bilateral ovariectomy, 14 weeks later, we measured bone mineral density(BMD) by dual-energy X-ray and determined that OP model was successfully replicated, OVX group rats were then divided into OVX group (n=15) and OVX+TFRD group (n=15). The OVX+TFRD group was given TFRD for 12 weeks. Glutamate (Glu), metabotropic glutamate receptor 5 (mGluR 5), and Glutamate/Aspartate Transporter (GLAST/EAAT 1)’s expression of femur was examined in order to clarify the characteristics of bone glutamate signaling pathway and the effects of TFRD on it. Results Glu and ionotropic receptors mGluR 5 mainly distributed in bone marrow cells and osteoblasts closed to the bone marrow cavity walls. There were no significant differences in Glu expression among Sham group, OVX group and OVX+TFRD group. The mGluR 5 expression of OVX+TFRD group was significantly higher than that of Sham group and OVX group(P=0.009), while no significant difference was found between the latter two groups. In addition to large distribution in bone marrow cells, small amount of transporter EAAT 1 was noted to express in bone cells of the bone lacunae. There were no significant differences in EAAT 1 expression among the three groups. Conclusion In bone glutamate signaling pathway, this study demonstrated that TFRD could significantly improve the ionotropic receptor mGluR 5’s expression, but had no inlfuence for Glu and EAAT 1.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第2期9-12,16,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
国家自然科学基金(81173619)
关键词
骨质疏松症
去卵巢大鼠
骨碎补总黄酮
谷氨酸
代谢型Glu受体-5
osteoporosis
ovariectomized rat
total flavonoids of rhizoma drynariae
glutamate
metabotropic glutamate receptor 5
