摘要
2011年6月4日,山东淄博某鸡场送检病例,该养殖场养殖规模8万羽,主要品种为乌鸡、麻鸡和海兰褐。乌鸡和麻鸡饲养在同一鸡舍,各4000只。患病乌鸡与麻鸡在360日龄时,表现为消瘦、嗜睡、鸡冠稍苍白,死亡率达10%左右,另一鸡舍的海兰褐鸡并无发病状况。通过大体剖检、组织病理学观察病变、血涂片观察血象,并利用PCR技术鉴定病鸡病毒感染情况,分离病毒,对其进行测序,分析病毒与参考毒株的同源性及变异情况。大体剖检可见肝、肾肿大,胸肌出现纤维肉瘤,肠管萎缩液化,胰出现黄豆大小的肿瘤结节;病理组织学观察发现乌鸡肝出现髓细胞瘤,腿部形成动脉血管瘤,肠管及胰腺间出现髓细胞瘤及血管瘤等,麻鸡肝出现成红细胞瘤,肺出现海绵状血管瘤。血涂片观察发现,血液中出现髓细胞样瘤细胞,中性粒细胞的数量和比例明显升高。病原学检测发现,ALV p27抗原和抗体检测均为阳性,PCR技术鉴定2种鸡均为ALV-J感染。分别从乌鸡和麻鸡中分离出1株和2株病毒,与各参考毒株比较显示,gp85区氨基酸的相似性为82.3%~91.1%,基因变异性较大,说明混养鸡群感染ALV-J后,不同宿主交叉感染,可能会促使ALV-J进化。
In June of 2011,sick chickens from a commercial flock at Zibo in shandong Province were sent to our lab for inspection.The flock size is 80 000.Main species breed in the farm were Black-bone silk fowl(BSF),Ma-chicken and Hy-Line chicken.The BSF and Ma-chicken were reared in the same house,4 000 each.The chickens were 360-day-old,and showed emaciation,listlessness,lethargy,pale comb.The mortality rate was about 10%.Hy-Line chickens in another house were not infected.Six of sick chickens were tested by gross,histopathology,blood smears observation and RT-PCR.The sequences of isolated viruses were analyzed by software.Necropsy observation showed enlargement of the liver and kidney,fibrosarcoma in chest,intestinal atrophy and liquefaction,soybean size of tumor nodules in pancreas.Histopathology observation showed that myelocytoma presented in the liver and arterial,and aneurysm existed in the leg.Fibrosarcoma appeared on the pectorales in BSF.Erythroblastoma of liver,spongy hemangioma of lungs were showed in Ma-chickens.Blood smear observation showed the occurrence of myeloid cell-like tumor cells,the number of neutrophils was significantly increased.Pathogen detection showed that ALV p27 antigen and antibody were both positive.The two kinds of chickens were both ALV-J positive in detection of RT-PCR.One and two strains of ALV-J were isolated from BSF and Ma-chicken,respectively.Comparing with the reference strains,the homologies of gp85 protein amino acid sequence were ranged from 82.3%-91.1%,suggesting greater genetic variability.It indicated that different host cross infection might happen and promote the rapid evolution of virus when mixed chickens infected with ALV-J.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第1期107-115,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31072096)
山东省农业重大应用科技创新课题(31700)
