摘要
Objective:To investigate in vitro larvicidal and antioxidant enzymes potential of the medicinal plants Ginkgo biloba（G.biloba）,Stevia rebaudiana（S.rebaudiana） and Parthenium hysterophorous（P.hysterophorous） against Anopheles stephensi（An.stephensi） 4th instars larvae. Methods:For evaluation of larvicidal potential,the ethanolic,methanolic and dichloromethane leaves extracts of three different plants were used in dose-dependent experiments in two media, while the antioxidant enzymes activities were investigated using four different methods viz., superoxide dismutase,peroxidase,ascorbate and catalase.Results:An.stephensi has developed resistance to various synthetic insecticides,making its control increasingly difficult.The comparative performance of ethanolic extracts（65%-90%） was found better than the methanolic extract（70%-87%） and dichloromethane extract（60%-70%）.Among the three plants extracts tested in two media,S.rebaudiana exhibited higher larvicidal activity with LC<sub>50</sub>（24 h） in methanolic extract than P.hysterophorous and G.biloba.G.biloba and P.hysterophorous exhibited the strongest antioxidative enzymes activity and S.rebaudiana were less active and no significant difference was observed.Conclusions:These three plants exhibit larvicidal potential and can be further used for vector control alternative to synthetic insecticide due to eco-friendly and diseases control,furthermore these plant species have potent antioxidative enzyme activities, therefore,making them strong natural candidate particularly for diseases which are caused due to free radicals.
Objective:To investigate in vitro larvicidal and antioxidant enzymes potential of the medicinal plants Ginkgo biloba(G.biloba),Stevia rebaudiana(S.rebaudiana) and Parthenium hysterophorous(P.hysterophorous) against Anopheles stephensi(An.stephensi) 4th instars larvae. Methods:For evaluation of larvicidal potential,the ethanolic,methanolic and dichloromethane leaves extracts of three different plants were used in dose-dependent experiments in two media, while the antioxidant enzymes activities were investigated using four different methods viz., superoxide dismutase,peroxidase,ascorbate and catalase.Results:An.stephensi has developed resistance to various synthetic insecticides,making its control increasingly difficult.The comparative performance of ethanolic extracts(65%-90%) was found better than the methanolic extract(70%-87%) and dichloromethane extract(60%-70%).Among the three plants extracts tested in two media,S.rebaudiana exhibited higher larvicidal activity with LC_(50)(24 h) in methanolic extract than P.hysterophorous and G.biloba.G.biloba and P.hysterophorous exhibited the strongest antioxidative enzymes activity and S.rebaudiana were less active and no significant difference was observed.Conclusions:These three plants exhibit larvicidal potential and can be further used for vector control alternative to synthetic insecticide due to eco-friendly and diseases control,furthermore these plant species have potent antioxidative enzyme activities, therefore,making them strong natural candidate particularly for diseases which are caused due to free radicals.
