摘要
Objective:To explore effect of retinal stem cells(RSCs)combined with copolymer-1(COP-1)immunotherapy on optic nerve damage in glaucoma rat model.Methods:A total of 40 SD rats were selected for glaucoma model and were randomly divided into 4 groups to observe protective effects of RSCs transplantation combined with COP-1.Results:Brain-derived neurotrophic factor(BDNF)and insulin like growth factor-1(IGF-1)were either positive in retina of RSCs transplanted or COP-1 immunological treated rat.Positive rate of BDNF and IGF-1 and expression of mRNA and protein were significantly higher in RSCs transplantation combined with COP-1immunotherapy treated rats compared with the other 3 groups,in which amount of apoptotic RGCs was lowest.Conclusions:RSCs transplantation combined with COP-1 immunotherapy can promote the secretion of BDNF and IGF-1.They protect RGCs in glaucoma rats in coordination,significantly reduce the number of apoptosis RGCs so as to alleviate the optic nerve damage.It ponits a new research direction for treatment of glaucoma.
Objective:To explore effect of retinal stem cells(RSCs)combined with copolymer-1(COP-1)immunotherapy on optic nerve damage in glaucoma rat model.Methods:A total of 40 SD rats were selected for glaucoma model and were randomly divided into 4 groups to observe protective effects of RSCs transplantation combined with COP-1.Results:Brain-derived neurotrophic factor(BDNF)and insulin like growth factor-1(IGF-1)were either positive in retina of RSCs transplanted or COP-1 immunological treated rat.Positive rate of BDNF and IGF-1 and expression of mRNA and protein were significantly higher in RSCs transplantation combined with COP-1immunotherapy treated rats compared with the other 3 groups,in which amount of apoptotic RGCs was lowest.Conclusions:RSCs transplantation combined with COP-1 immunotherapy can promote the secretion of BDNF and IGF-1.They protect RGCs in glaucoma rats in coordination,significantly reduce the number of apoptosis RGCs so as to alleviate the optic nerve damage.It ponits a new research direction for treatment of glaucoma.
基金
supported by Shanghai Science and Technology Commission Project Fund(No 11ZR14121300)
作者简介
Corresponding author:Lin Zhang,E-mil:28906390@qq.com
