摘要
采用烟草靶斑病菌(Rhizoctonia solani)粗毒素原液处理6叶期烟草幼苗,测定12、24、36、48、60、72h后烟草叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)的活性和丙二醛(MDA)含量。结果表明,经烟草靶斑病菌粗毒素处理后的烟草POD、PAL的活性及MDA含量都高于对照,并呈现一定的波动性;PPO和CAT先升高后下降,始终高于对照;而SOD酶的活性表现为持续下降。
Tobacco seedlings at 6-leaf stage were treated with crude toxin from tobacco target spot disease(Rhizoctonia solani),of which the peroxidase(POD),polyphenol oxidase(PPO),superoxide dismutase(SOD),catalase(CAT) and phenylalanine ammonialyase(PAL) activity as well as malondialdehyde(MDA) content was measured 12,24,36,48,60,72 h after treatment.The results demonstrated that after toxin treatment,POD,PAL and MDA were activated as their activities were higher than control,changes of POD,PAL and MDA were undulate;PPO and CAT activities increased firstly and then decreased along with time increasing,but always higher than control;SOD activity increased continually.
出处
《湖北农业科学》
北大核心
2013年第3期575-577,共3页
Hubei Agricultural Sciences
基金
国家烟草专卖局科技攻关项目(国烟办综[2010]182号)
辽宁省烟草专卖局科技攻关项目(辽烟计[2010]86号)
