摘要
目的：优化建立水产品中氧化三甲胺(trimethylamine-N-oxide, TMAO)离子色谱法测定的前处理方法,并对青岛地区常见水产品的氧化三甲胺含量进行测定。方法考察了三氯乙酸(TCA)法、Tris-TCA法、Tris-乙腈法三种常用的前处理方法对离子色谱测定结果的影响并进行了优化,收集了青岛常见水产中具有代表性的品种,采用非抑制性离子色谱法对氧化三甲胺含量进行了测定。结果测定了头足类、硬骨鱼类、贝类和甲壳类等21种青岛地区常见水产品中的氧化三甲胺含量。其中,阿根廷鱿鱼TMAO含量最高,为8791.89 mg/kg；硬骨鱼TMAO含量范围为350~2300 mg/kg；甲壳类除了刀额新对虾以外,含量均在1700 mg/kg之上；贝类TMAO含量均在500 mg/kg之下。结论所测海产品中,头足类、甲壳类的氧化三甲胺含量较高,贝类含量较低,大致呈现头足类>甲壳类>硬骨鱼类>贝类的趋势。
Objective The pretreatment procedure for trimethlylamine-N-oxide (TMAO) detection in aquatic products by ion chromatography was established, and TMAO contents of common aquatic prod-ucts from Qingdao local market was also measured in this study. Methods In this research, three pre-treatments based on trichloroacetic acid, tris and trichloroacetic acid, tris and methyl cyanide were com-pared to investigate the effect of them on ion chromatography determination. Some of the most common aquatic products in Qingdao were collected for TMAO contents determination, which was conducted by non-inhibitory ion chromatography. Results Twenty-one aquatic products commonly seen in Qingdao were analyzed, and the test samples consisted of teleost, crustacea, shellfish and cephalopod. The content of TMAO in Illex argentinuscastellanos was shown to be the highest (8791.89 mg/kg). TMAO contents varied from 350 mg/kg to 2300 mg/kg in teleost. All crustaceans except Metapenaeus ensi contained TMAO with concents over 1700 mg/kg. All shellfishes contained TMAO with contents under 500 mg/kg. Conclusion In the twenty-one aquatic products analyzed, TMAO contents were high both in cephalo-pods and crustaceans, while lower in shellfishes. The general TMAO content trend might be concluded as cephalopods>crustaceans>teleost>shellfish.
出处
《食品安全质量检测学报》
CAS
2014年第1期41-46,共6页
Journal of Food Safety and Quality
基金
"十二五"国家科技支撑计划项目(2012BAD33B07)
国家自然科学基金项目(31371757)~~
关键词
青岛
氧化三甲胺
水产品
离子色谱法
Qingdao
trimethylamine N-oxide(TMAO)
aquatic products
ion chromatography
