摘要
目的：建立固相微萃取-气相色谱-质谱联用仪测定醇食品模拟液中16种多环芳烃的分析方法。方法优化了萃取方式、萃取温度、萃取时间、解吸时间和振荡速率等萃取条件,样品采用气相色谱-质谱联用仪(gas chromatography-mass spectrometry, GC-MS)总离子流和选择离子模式进行定性定量测定。结果16种多环芳烃在0.01~10μg/L范围内线性良好, R2大于0.99。在0.1、0.5、1.0μg/L 3个添加水平下,16种多环芳烃的平均回收率为76.52%～119.8%,相对标准偏差为0.99%～15.93%。结论该方法操作简便、灵敏度高、准确度好,能够满足醇食品模拟物中多环芳烃化合物的检测要求。
Objective To develop a method for the determination of polycyclic aromatic hydrocarbons (PAHs) in alcohol food simulation by chromatography-mass spectrometry (GC-MS) using solid phase microex-traction (SPME). Methods The experimental conditions of SPME, such as extraction mode, extraction tem-perature, extraction time, desorption time and agitation speed were optimized. Qualitative and quantitative analysis were carried out by total ion chromatogram and selected ion chromatogram with GC-MS. Results The method showed a good linearity in the range of 0.01~10.00μg/L for PAHs with R2>0.99. Average recove-ries of PAHs were 76.52%~119.8%with relative standard deviation of 0.99%~15.93%at three fortification le-vels of 0.1, 0.5, and 1.0μg/L. Conclusion This method is simple, sensitive, and accurate, and it is suitable for the determination of PAHs in 10%alcohol food simulation.
出处
《食品安全质量检测学报》
CAS
2013年第5期1478-1484,共7页
Journal of Food Safety and Quality
基金
深圳出入境检验检疫局科研项目(SZ2010010)~~
关键词
多环芳烃
气相色谱-质谱法
醇食品模拟物
polycyclic aromatic hydrocarbons
gas chromatography-mass spectrometry
alcohol food simulation
