摘要
Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida. Methods Hypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase, and the detection wavelength was at 283 nm with flow rate at 1 mL/min. Results All calibration curves showed good linear regression (r > 0.9990) within the tested range. All average recovery was more than 98.00% and RSD was less than 3.0% (n = 6). Conclusion The method is steady and with good repeatability, and could be used to determine the content of flavonoids in A. frigida from different areas.
Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida. Methods Hypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase, and the detection wavelength was at 283 nm with flow rate at 1 mL/min. Results All calibration curves showed good linear regression (r > 0.9990) within the tested range. All average recovery was more than 98.00% and RSD was less than 3.0% (n = 6). Conclusion The method is steady and with good repeatability, and could be used to determine the content of flavonoids in A. frigida from different areas.
