摘要
该文采用高效液相色谱法(HPLC)测定不同海拔与不同保存时间下重庆产青蒿中青蒿素的质量分数,通过生物学统计法分析其质量分数与药材保存时间或种植海拔之间可能存在的相关性,从而探讨其有关的质量变化规律,为选取重庆产青蒿最佳的种植海拔和保存时间提供科学依据.结果显示,采集的12个重庆产青蒿样品中青蒿素平均质量分数为0.434 8%,质量分数范围在0.254 3%~0.562 0%之间;其中不同海拔下的青蒿中青蒿素质量分数差异显著(p<0.05),以种植在海拔900m左右青蒿的青蒿素质量分数最高,为0.444 3%;不同保存时间下青蒿中青蒿素质量分数差异显著(p<0.05),以采收后立即入药的青蒿中青蒿素质量分数最高,同时随其保存时间的延长青蒿素质量分数呈下降趋势.重庆产青蒿最适种植高度在海拔900m左右;采收后不宜长时间保存,应当及时入药.
The effect of different elevations and preservation on contents of Chongqing Artemisia annua L by HPLC has been studied.We can provide the references to the planting location and quality research of Chongqing authentic Artemisia annua L.The result shows that the 12 samples from Chongqing Artemisia annua L average contents Artemisinin is 0.4348% and all the samples Artemisinin between 0.254 3%-0.562 0%.The different elevations have a significant effect on determination of Artemisinin in the extacts of Chongqing Artemisia annua L(p<0.05),and the best elevation is 900m.The different holding period have a significant influence(p<0.05),the longer holding period,the lower contents.The conclusion is that the optimal planting location of Chongqing Artemisia annua L is ascertained at altitude 900 meters;and used as medicine immediately.
出处
《西南师范大学学报(自然科学版)》
CAS
CSCD
北大核心
2012年第12期83-87,共5页
Journal of Southwest China Normal University(Natural Science Edition)
基金
重庆市科委攻关资助项目(2011GGC009)
关键词
重庆
青蒿
青蒿素
Chongqing
Artemisia annua L
Artemisinin
作者简介
谢光武(1985-),男,重庆江北人,硕士研究生,主要从事中药质量控制研究.;