摘要
目的:观察蝙蝠葛酚性碱(PAMD)的抗肿瘤作用并进一步探讨其作用机制。方法:通过实时定量PCR(Real-time PCR)技术观察PAMD对胰腺癌细胞株BxPC-3皮下异位移植瘤细胞凋亡相关基因p53mRNA表达的影响,以此探讨PAMD抗肿瘤作用机理。结果:PAMD能够显著降低胰腺癌细胞株BxPC-3裸鼠皮下异位移植瘤组织中p53 mRNA表达量。结论:PAMD对胰腺癌细胞株BxPC-3裸鼠皮下异位移植瘤表现出明显抑制作用,能够有效的抑制肿瘤生长,其机理可能与凋亡相关基因表达有关。
Objective:To study and explore the anti-tumor effects and the further mechanism of phenolic alkaloids from Menispermum dauricum(PAMD)on subcutaneously heterotopic transplantation tumor in nude mouse from pancreatic carcinoma cell BXPC-3.Methods:Real-time polymerase chain reaction were used to observe the mRNA expression of apoptotic related gene p53.Results:PAMD can efficiently decrease the expressions of p53 mRNA on subcutaneously heterotopic transplantation tumor from pancreatic carcinoma cell BXPC-3.Conc...
出处
《中医药学报》
CAS
2009年第6期35-37,129-130,共5页
Acta Chinese Medicine and Pharmacology
基金
2007黑龙江省自然科学基金课题(编号:D200675)
关键词
蝙蝠葛酚性碱
荷瘤小鼠
P53
Phenolic Alkaloids from Menispermum Dauricum（PAMD）
Anti-tumor
p53
