摘要
目的:建立血浆中拉贝洛尔的反相高效液相色谱-荧光检测法。方法:血浆样品经叔丁基甲醚一次萃取后有机相挥干,以少量流动相重组,采用KromasilKR100-5C1(8250mm×4.6mm,5μm)为分析柱,甲醇:水:2.4molL二乙胺-磷酸缓冲液(pH=4.0)(50:48:2)为流动相,荧光激发波长(Ex)为302nm,发射波长(Em)为420nm。拉贝洛尔和内标(盐酸普萘洛尔)的保留时间分别为5.5min和8.0min。结果:血浆中的拉贝洛尔在2.5ngmL～160ngmL范围内线性关系良好,r=0.9998。方法的最低检测浓度为2.5ngmL,样品和内标平均提取回收率分别为81.28%和88.68%,批内、批间精密度为0.42%～7.72%。结论:本方法灵敏可靠,可满足拉贝洛尔的临床血药浓度监测和药代动力学研究的需要。
Objective:To develop a high performance liquid chromatographic method with fluorimetric detection for the determination of labetalol in plasma.Methods:The plasma sample was treated with tert-Butyl Methl Ether extract.The HPLC method was performed on a column of Kromasil KR100-5C18(250mm×4.6mm,5μm)with the mobile phase of methanol-water-2.4mol L diethylamine phosporic acid(50:48:2),and the detective wavelength at Ex=302nm and Em=420nm.The retention time of labetalol and IS(proparnolol)were 5.5min and 8.4min respectively.Results:Labetalol was linear from 2.5ng mL to 160ng mL(r=0.9998).The extraction recovery of labetalol and IS was 81.28% and 88.68%.The limit of detection is 2.5ng mL.The inter-day and intro-day RSDs were 0.42%～7.72% respectively.Conclustion:This method is sensitive and can be used for TDM and pharmacokinetic studies of labetalol.
出处
《中国药物应用与监测》
CAS
2005年第3期18-20,共3页
Chinese Journal of Drug Application and Monitoring
作者简介
姚蓝,女,主管药师,主要从事药物分析方面的研究和工作Tel:(021)54030254
