摘要
目的研究卵巢上皮癌组织中T淋巴细胞、树突状细胞数量及分布,为进行卵巢癌生物治疗提供依据。方法采用ABC免疫组织化学和图像分析技术,测定人卵巢上皮癌组织和正常卵巢组织中T细胞表面分化抗原CD3、CD4、CD8,树突状细胞表型抗原CD1c、S-100阳性表达的细胞及其表达强度。结果 与正常卵巢组织相比,卵巢上皮癌中CD3+、CD8+、CD1c+、S—100+细胞数量明显减少,其表达强度明显降低,差异有显著性(P<0.05)。卵巢上皮癌中CD4+抗原阳性表达细胞数量明显减少,但其表达强度变化不明显。结论 卵巢上皮癌组织T淋巴细胞、树突状细胞数量及其表达强度发生变化,提示卵巢癌局部免疫发生改变。
Objective To investigate the number, distribution, expression intensity of T lymphocytes and dendritic cells in human ovarian epithelial carcinoma for seeking biotherapeutic evidence of ovarian carcinoma. Methods Using ABC immu-nohistochemical and computerized imaging technique, to detect the number, distribution, and expression intensity of CD3, CD4, CD8,CD1c,S- 100 positive cells in ovarian epithelial carcinoma.Results Compared with normal ovarian tissue, the number and the expression intensity of CD3+ , CD8+ , CD1c+, S - 100+ cells in ovarian carcinoma significantly. Hecreased. Although the number of CD4+ cells in ovarian epithelial carcinoma reduced significantly. However, no significant difference in its expression intensity was found. Conclusions The number and the expression intensity of T lymphocytes and dendritic cells in human ovarian epithelial carcinoma had changed. The results suggest that there are some changes in local immunologic function in human ovarian epithelial carcinoma.
出处
《中国妇产科临床杂志》
2002年第1期48-50,共3页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
国家自然科学基金资助(项目编号No.30070843)
