摘要
对目前文献所见到的植酸酶活的测定方法选取三种进行了分析和修正,并用两种温度下的酶活定义检验修正过的测定方法的差异。根据显色剂吸收光谱和磷化合物的吸收光谱的比较,确定FeSO_4—钼蓝法选取大于720nm、VC—钼蓝法选取620～660nm、丙酮法选取400～420nm的波长为测量适宜波长。在同样温度下(即对一种酶活定义来说,)用这三种方法测定曲霉发酵液的植酸酶活,结果酶活大小基本一致(平均差率小于5％),说明这三种方法在测定植酸酶活时都有相等的可靠性和准确性。
Several measure methods of phytase activity units in recent literature has been analyzed and modified, they also were used to test the accuracy of two definition of phytase activity. From the color development reagent and phosphorus complex absorption spectrum, the best condition to measure free phosphorus by phytase. more than 720nm wavelength should be used in FeSO_4— molybdnum blue method, 620～680nm in VC-molybdnum blue method, and 400～420nm in acetone method. The value of the three measure methods of phytase activity were the same at the same temperature (average deviation < 5%), it means that all the three methods were the same accuracy.
出处
《福建分析测试》
CAS
2002年第2期1564-1567,共4页
Fujian Analysis & Testing
基金
福建省自然科学基金重点项目(D0120002)
关键词
植酸酶活
测定
吸收光谱
Phystase activity
Measurement
Absorption spectrum
作者简介
彭益强(1973-),男,硕士,从事生物工程研究.
