摘要
2005年以来,在广东、广西地区出现了大面积桉树商品人工林无性系生长停滞的现象,为了确定该病症是否由植原体侵染引起,用直接PCR和巢式PCR扩增植原体16SrDNA的方法对具有典型症状的样品进行了植原体检测,结果并没有检测到植原体的存在。
Since 2005,large area of commercial eucalypt plantations have exhibited so called slow-growing symptoms in Guangdong and Guangxi.The study was carried out to confirm whether this disease is caused by phytoplasma.Both direct-PCR and nest-PCR were employed to detect the phytoplasma by cloning 16 SrDNA from samples with typical symptoms.The results showed that there was no phytoplasma detected from the samples indicated phytoplasma is not the causal agent of the disease.
出处
《桉树科技》
2009年第1期36-39,共4页
Eucalypt Science & Technology
基金
国家"十一五"科技支撑专题(2006BAD08A11)
中央级公益性科研院所基本科研业务费专项资金(CAFYBB2007024)
科技部国际合作项目(2007DFA31190)
关键词
桉树
滞长症
植原体
PCR
Eucalyptus spp.
slow-growing
Phytoplasma
PCR
