摘要
目的研究过氧化物酶体增殖物激活受体γ(PPARγ)和肿瘤抑制基因PTEN在肝癌组织中表达的相关性,以及两种基因对人肝癌细胞BEL-7402细胞生长的影响,并探讨其机制。方法分别应用RT-PCR和Western blot观察分析肝癌组织、癌旁组织以及肝癌细胞中PPARγ和PTEN的表达情况;应用MTT法观察PPARγ的合成配体罗格列酮(RGZ)对BEL-7402细胞生长的影响。结果PPARγ mRNA和PTEN mRNA在肝癌组织较癌旁组织中表达是显著降低的,两者在肝癌组织中的表达呈正相关(r=0.774,P<0.01)。罗格列酮呈剂量和时间依赖性关系诱导PTEN蛋白的表达和抑制BEL-7402细胞的生长,此效应是通过PPARγ通路活化实现的。结论PPARγ和PTEN在肝癌中表达呈正相关,RGZ能够抑制BEL-7402细胞的生长,此效应与RGZ诱导PPARγ通路活化和PTEN表达上调有关。
Objective To investigate the correlation of PPARγ and PTEN mRNA expressions of hepatocellular carcinoma(HCC) tissue samples and the effect of both genes on human HCC cell line BEL-7402 cell growth.Methods RT-PCR analysis was performed to detect the mRNA expression levels of PPARγ and PTEN in 21 HCC samples.The expression of PTEN protein were assessed by Western blot analysis.The cell growth status of BEL-7402 cells was evaluated by MTT assay.Results The mRNA expression of PTEN and PPARγ decreased in HCC tissues,compared with adjacent non-tumor liver tissues,and both the mRNA expressions indicated positive correlation(r=0.774,P<0.01).Rosiglitazone,as a synthetic ligands of PPARγ,inhibited BEL-7402 cell growth and increased PTEN protein expression by PPARγ pathway.Conclusion PTEN and PPARγ gene expression present positive correlation in HCC tissues.Rosiglitazone activate PPARγ pathway to increase PTEN expression,which may be the molecular mechanism of rosiglitazone-inhibited BEL-7402 cell growth.
出处
《中华普通外科学文献（电子版）》
2007年第3期136-138,共4页
Chinese Archives of General Surgery(Electronic Edition)
关键词
过氧化物酶体增殖物激活受体
PTEN
肝癌
Peroxisome proliferator-activated receptor γ
PTEN gene
Hepatocellular carcinoma
作者简介
汪谦,电子邮箱:wangqian00@hotmail.com 通讯作者
Corresponding author: WANG Qian, E-mail:wangqian00@hotmail.com
