摘要
从感染致弱的Ⅰ型马立克氏病病毒（ＭＤＶ）８１４株的细胞中提取病毒基因组ＤＮＡ，以ｄｉｇｏｘｉｎｇｅｎｉｎ标记ＭＤＶ强毒ＧＡ株糖蛋白Ｂ（ｇＢ）基因片段作为探针，进行Ｓｏｕｔｈｅｒｎ－ｂｌｏｔ杂交定位。将包含ＭＤＶ ８１４株ｇＢ基因的基因组片段克隆于ｐＵＣ１９质粒中。酶切鉴定后，进行序列分析。结果表明该 ｇＢ基因具有高度的保守性，编码区序列同已发表的Ⅰ型马立克氏病病毒 ｇＢ基因序列完全一致，但启动子上游区域有差异。
The genomic DNAs of the strain 814 of Marek s disease virus(MDV) were extracted from chickes embryo fibroblasts infected by the strain 814 of MDV. The gene encoding glycoprotein B (gB) of the strain 814 of MDV was oriented by southern-blot. Futhermore, the gB was cloned into plasmid pUC19, and the entire nucleotide sequence of the gene was determined. The results indicated that gB of MDV type Ⅰ was highy conservative, and the encoding sequence of gB of strain 814 was the same as that of strain RBIB of MDV, ...
出处
《中国预防兽医学报》
CAS
CSCD
2000年第S1期28-31,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家863计划资助项目
