摘要
目的观察吡格列酮对缺血再灌注损伤心肌细胞的保护作用,为吡格列酮治疗心肌缺血再灌注损伤提供理论基础和实验依据。方法体外培养Wistar大鼠乳鼠心室肌细胞,建立心肌细胞缺氧复氧模型。比色法检测各组培养基中SOD活力和MDA的含量;采用碘化丙啶标记流式细胞术和Hochest33258免疫荧光法检测心肌细胞凋亡率。结果吡格列酮使培养的缺血再灌注损伤心肌细胞培养基中的MDA含量降低;SOD活力升高(P<0.05);凋亡指数显著降低。结论吡格列酮减轻离体缺血再灌注损伤心肌细胞凋亡,具有保护作用。
Objective To reseach the protective effect of pioglitazone on myocardial cells in vitroto with ischemia reperfusion injury and to provide theoretical basis and experiment evidence for the treatment of myocardial cells with ischemia reperfusion injury.Methods In this experiment,newborn wistar rats hearts were isolated and their cardiomyocytes were separated using trypsin digestion method.The hypoxia reoxygenation injury model was established by 3 hours of hypoxia and subsequently 3 hours of reoxygenation.The...
出处
《潍坊医学院学报》
2008年第6期-,共4页
Acta Academiae Medicinae Weifang
基金
山东省教育厅资金资助(J07YE01)
关键词
心肌细胞
吡格列酮
缺血再灌注损伤
凋亡
Cardiomyocyte
Pioglitazone
Ischemia reperfusion injury
Apoplosis
