摘要
目的研究一种本室设计的B型CpG ODN(BW006)作为乙肝疫苗佐剂的可行性。方法以琼脂糖凝胶电泳法对BW006的质量进行初步检定;H3-掺入法检测BW006对小鼠脾细胞的刺激作用;乙肝疫苗与BW006联合免疫Balb/c小鼠,并用ELISA法检测小鼠血清中的HBsAb水平。结果BW006的纯度和完整性符合实验要求,并能显著的刺激小鼠脾细胞的增生,同乙肝疫苗联用后能够明显增强乙肝疫苗的免疫效果。结论BW006对乙肝疫苗有较强的免疫佐剂作用。
Objective To identify the adjuvant effects of CpG ODN(BW006) which was designed by our laboratory.Methods Agarose gel electrophoresis was used to identify the quality of BW006;the activity effect of BW006 on mouse splenocyte was detected by H3-incorporated method;Balb/c mice were immunized with hepatitis B vaccine and BW006,and then the level of HBsAb in mouse serum was assayed by ELISA.Results The purity and integrity of synthesized BW006 was qualified.This CpG ODN could significantly activate mouse spleno...
出处
《免疫学杂志》
CAS
CSCD
北大核心
2009年第1期52-55,共4页
Immunological Journal
基金
国家863计划项目(2006aa02z487)
