摘要
目的:研究Aβ1-40诱导阿尔茨海默病(Alzheimer’s disease,AD)大鼠空间学习记忆能力及海马区星形胶质细胞(Astro-cyte,AS)胶质纤维酸蛋白(Glial fibrillary acidic protein,GFAP)表达及电刺激小脑顶核(Fastigial nucleus stimulation,FNS)的干预作用。方法:将Aβ1-40微量注射至大鼠右侧海马,制作AD大鼠模型,干预组给予电刺激小脑顶核和电刺激齿状核(Dentate nucle-us stimulation,DNS),28d后进行水迷宫试验。免疫组织化学法检测GFAP免疫阳性细胞表达。结果:FNS干预后AD大鼠空间学习记忆能力明显改善(P<0.05)。与假手术组相比,AD组、DNS组大鼠脑组织海马区可见星形胶质细胞GFAP表达明显增多(P<0.05),FNS组大鼠脑组织海马区星形胶质细胞GFAP表达较AD组下降(P<0.05)。结论:FNS可部分改善Aβ1-40诱导的AD模型大鼠空间学习记忆障碍,其机制可能与降低GFAP的表达和抑制AS活性有关。
Objective:Observe the effects of fastigial nucleus stimulation(FNS) on spatial memory and learning ability of the Aβ1-40 induced Alzheimer rat model,by detecting GFAP expression of hippocampus area. Methods:Aβ1-40 was microinjected into the right hippocampus of rats. Cerebella dentate nucleus and fastigial nucleus were stimulated separately before the model was made in interference group. The spatial memory and learning ability of the rat was evaluated by the Morris water maze procedure at the 28th days after injection. The expression of GFAP in astrocyte cell was determined with immunohistochemistry method.Results:The AD rats received FNS showed significant shorter escape latency than the group without FNS(P<0.05).Aβ1-40 could increase the optical density of positive cells of GFAP(P<0.05),FNS could reduce the optical density of positive cells of GFAP(P<0.05),but in dentate nucleus stimulation group no such effect was found. Conclusion:FNS can partly improve the spatial learning and memory dysfunction of AD rat by inhibits the expression of GFAP of hippocampus area and decrease the activity of astrocyte cell.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第z1期52-54,66,共4页
Journal of Chongqing Medical University
作者简介
罗晶(1983-),女,硕士,研究方向:老年痴呆
王永红,女,教授,E-mail:wyh0231029@yahoo.com.cn. 通讯作者
