摘要
对沙田柚茎尖嫁接苗的离体培养进行了研究，结果表明：顶芽在Ｍｓ十０．５ｍｇ／Ｌ６－ＢＡ上生长较好，成活率为１００％，单个外植体平均不定芽数为２．２，但不定芽生长极慢，成苗困难。加入０．２－０．５ ｍｇ／ＬＩＢＡ，顶芽生长加快，但单个外植体平均不定芽数下降。带有１ ｃｍ长积壳砧木的茎尖在ＭＳ十０．５ｍｇ／Ｌ６-ＢＡ上可以形成丛芽，单个外植体平均不定芽数为４．５，而且不定芽生长迅速。虽然我们未能诱导外植体不定根发生，但通过试管嫁接可以获得完整植株。
The culture of shoot-tip grafting plantlets of Shatian pomelo had been studied in vita. The results showed the shoot-tips growed better in MS + 6-BA 0.5 mg/L, the survival percentage got100%, and the average adventitious shoots per explant were 2.2, but the average adventitious shoots growed slowly, they were very difficult to form full plantlets. The shoot-tips growed rapidly if we added 0.2 .5 mg/ IBA into MS + 6 BA 0.5 mg/L, but the average adventitious shoots per explant decreased. The shoot tips of Shatian pommelo with 1 cm stock of citrange could form the clump-shoots, their survival percentage also got 100%, the average adventitious shoots per explant were 4.5, and the average adventitious shoots cauld grow rapidly. We could acquire the full plantlets via the tube-grafting although we didn?t induce the generation of adventitious mats.
出处
《生态科学》
CSCD
2001年第3期14-19,共6页
Ecological Science
基金
国家自然科学基金资助项目(39870539)
广东省自然科学基金资助项目(980478)
广东省创新百项工程资助项目(99)
关键词
沙田柚
茎尖嫁接苗
离体培养
不定芽
Citrus grandis Osbeck (Shatian pomelo)
shoot-tip grafting plantlet
culture in vitro
adventitious shoot
作者简介
董高峰(1971-),男,博士生;主要从事植物细胞工程研究工作.
