摘要
目的:研究严重急性呼吸综合征患者的病情、病程和特异性抗体产生对SARS病毒基因阳性检出率的影响。方法:采用巢式反转录PCR检测健康人,发烧非SARS患者,不同时期和不同病情SARS患者以及存不存在特异性抗体的SARS患者痰标本中的SARS病毒核酸即RNA。结果:巢式RT—PCR检测SARS患者痰标本中的SARS病毒RNA的敏感度为60.0％。6 d～10 d组阳性检出率最高,为100％,高于11 d～25 d组,而11 d～25 d组的阳性检出率又高于>25 d组(P<0.01);病情轻组比病情重组的阳性检出率高(P<0.05);抗体阴性组比抗体阳性组检出率高(P<0.05)。结论:SARS病毒基因检测阳性率在轻病情组比重病情组高,住院时间短组比住院时间长组高,血清特异性抗体阴性组比阳性组高。
Objective to investigate the relation between the positive detection rate of the SARS-associated coronavirus and the severity of SARS symptoms, the course and the levels of specific antibody. Methods the SARS-associated coronavirus RNA in the sputum of the healthy, feverous patients without SARS, the SARS patients in diffrernt course and severity, and with or without specific antibody were detected by nested RT-PCR. Results the sensitivity of the SARS-associated coronavirus RNA in the sputum of SARS patient...
出处
《实用医技杂志》
2004年第6期676-677,共2页
Journal of Practical Medical Techniques
