摘要
目的 :应用红外光谱法研究多味中药混合水提液对肝癌细胞的作用机制。 方法 :用傅立叶变换红外光谱法 (FTIR)研究肝癌 SMMC- 772 1细胞 (空白组 )及其经夏枯草、鱼腥草、柴胡、茵陈等 4味抗癌中药水提液培养 (中药组 ) 2 0 h后的红外光谱 ,考察光谱上表征蛋白质的酰胺带、核酸的磷酸二酯带等的峰形、峰位、峰强变化 ,以及部分特征峰峰强比的变化 ,并对各红外光谱进行聚类分析。 结果 :中药组与空白组的红外光谱在峰形、峰位、峰强度等均存在不同程度的差异 ,中药组的 υs(PO2 - )、υas(PO2 - )谱带多向低波数移动 ,υas(CH3)、δ(CH2 )谱带多向高波数移动。中药组的υas(CH3)与υs(CH2 )相对峰强比减小 ,υs(PO2 - )与 δ(N- H)相对峰面积比值大多升高 ,分别提示对 SMMU- 772 1细胞中膜结构的破坏作用、对 DNA复制的抑制作用等。聚类分析将中药组与空白组分开 ,不归肝经的中药与归肝经的中药分开。结论 :通过 FTIR可以对由中药作用引起的细胞变化进行快速、有效的分析 ,对阐明中药的作用机制有提示作用。
Objective:To study the effects of several herbal medicines on SMMC-7721 liver cancer cells with Fourier transform infrared spectrometer(FTIR). Methods: FTIR was employed to determine the infrared spectra(IRs) of SMMC-7721 liver cancer cells cultivated for 20 h with the extracts of Spica prunellae, Herba houttuyniae, Radix bupleuri and Herba artemisiae scopariae. Cluster analysis of IRs was also performed. Results: IR spectral parameters such as band shape, intensity and frequency of the blank, control and herbal-extract-treated cells were compared. There existed obvious blue shift of υ s(PO 2 -), υ as (PO 2 -) bands, red shift of υ as (CH 3), δ(CH 2) bands on the herbal-extract-treated cells IRs. The decreasing ratio of υ as (CH 3) to υ s(CH 2) peak intensity and the increasing ratio of υ s(PO 2 -) to δ(N-H) peak area indicated the destructive effect of herbal extracts on the membrane structure of SMMU-7721 cells and inhibitory effect on the DNA replication respectively. Cluster analysis successfully discriminated the herbal-extract-treated cells from the blank cells and the liver-oriented medicines from the non-liver-oriented medicine. Conclusion: FTIR provides another fast and effective approach to analyze the changes of cells treated with Chinese herbal medicines, which may help to illuminate the functional mechanism of Chinese herbal medicines.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2004年第10期1100-1103,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金 ( 3 0 10 0 2 3 3 )
作者简介
陆峰(1971-),男(汉族),博士,副教授.陆峰,Corresponding author.E-mail:lookingll@etang.com
