摘要
目的研究并测定三七主根和根茎中三七皂苷R1和人参皂苷Rg1、Rb1的含量及指纹图谱。方法采用RP-HPLC法,色谱柱为Phenomenex C18(150 mm×4.60 mm,5μm);流动相为乙腈-水(梯度洗脱);流速1.0 ml.min-1;检测波长203nm。结果含量测定中三七皂苷R1和人参皂苷Rg1、Rb1的线性范围分别为0.184~1.840、0.994~9.940、0.896~8.960μg(r=0.9999),平均回收率分别为99.7%(RSD=1.21%)、101.3%(RSD=1.32%)、100.7%(RSD=0.93%)(n=9)。三七主根和根茎的HPLC指纹图谱分离效果好。结论所建含量测定和指纹图谱的方法简便、准确,重复性好。测定结果显示,无论是三七各成分的含量,还是色谱峰的数目,根茎均明显高于三七主根。
OBJECTIVE To develop a method for the determination of sanchinoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Radix Notoginseng and its extractions and to study the fingerprint in Radix Notoginseng and its extractions.METHODS An RP-HPLC method was adopted.The analysis was carried out on an analytical column C18(150 mm×4.60 mm,5 μm).The flow rate was 1.0 ml·min-1 and the detective wavelength was set at 203 nm.The mobile phase consisted of acetonitrile-water(gradient elution).RESULTS The linear range was 0.18...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第3期332-334,共3页
West China Journal of Pharmaceutical Sciences
关键词
三七主根
三七根茎
三七皂苷R1
人参皂苷RG1
人参皂苷RB1
含量测定
指纹图谱
Radix Notoginseng taproot
Radix Notoginseng Rhizome
Sanchinoside R1
Ginsenoside Rg1
Ginsenoside Rb1
Content determination
Fingerprints
