摘要
目的研究miRNA22-3p、miRNA223-3p在痛风性关节炎患者中的表达水平及其炎症免疫反应调节机制。方法2020年1月到2021年1月在秦皇岛市第一医院诊治且符合痛风诊断标准的80例痛风患者资料纳入研究作为观察组进行回顾性分析。同期行健康体检且临床指标无明显异常,身体健康的80例患者作为对照组。排除标准:合并重要脏器系统严重疾病者;近3个月使用过激素类药物者;其他疾病所致继发性痛风性关节炎;药物诱发痛风性关节炎发作者;精神障碍疾病者等。采用独立样本t检验比较两组的miRNA22-3p、miRNA223-3p、炎性反应以及免疫指标之间的差异;Pearson相关性分析miRNA22-3p和miRNA223-3p与炎性因子的关系。结果观察组患者的miRNA22-3p、miRNA223-3p水平分别为(4.0±1.0)、(1.51±0.12),显著高于对照组的(1.12±0.26)、(0.62±0.20)(t=24.333、34.130,均为P<0.05)。观察组患者的白介素(IL)-1β、肿瘤坏死因子(TNF)-α及IL-6水平分别为(57.77±7.24)、(24.04±4.24)、(49.27±3.72)均显著高于对照组的(42.27±3.31)、(18.07±4.17)、(39.75±3.73)(t=17.415、8.979、16.164,均为P<0.05);观察组的单核细胞趋化因子(MCP)-1、胸腺调节趋化因子(TARC)、嗜酸性粒细胞趋化因子(eotaxin)-2水平分别为(106±12)、(215±13)、(347±11)均显著高于对照组(72±12)、(159±12)、(317±12)(t=18.095、29.356、16.713,均为P<0.05)。Pearson相关性分析显示患者的miRNA22-3p、miRNA223-3p与MCP-1、TARC、eotaxin-2、IL-1β、TNF-α以及IL-6呈现显著正相关(r=0.609、0.659、0.545、0.610、0.551、0.571、0.684、0.668、0.671、0.542、0.534、0.458,均为P<0.05)。结论miRNA22-3p、miRNA223-3p在痛风性关节炎患者中的表达水平显著升高,与炎症以及免疫反应呈现正相关。
Objective To investigate the expression levels of miRNA22-3p and miRNA223-3p in gouty arthritis patients and the regulatory mechanism of inflammatory immune response.Methods The data of 80 gout patients who met the gouty diagnosis creteria and treated in the First Hospital of Qinhuangdao from January 2020 to January 2021 were retrospectively analyzed.Other 80 healthy patients who underwent rountine health check-up during the same period without obvious abnormality in clinical indicators were enrolled as the control group.Exclusion criteria:the patients with severe diseases of the vital organs and systems;patients who used hormone drugs in the past three months;secondary gouty arthritis caused by other diseases;dryg induced gouty arthritis attacks;mental disorders.The observation group met the diagnostic criteria of gout.Independent sample t test was used to compare the differences in miRNA22-3p,miRNA223-3p,infla-mmatory response and immune indicators between the two groups.Pearson correlation analysis was applied for the relationship between inflammatory indicators and miRNA22-3p as well as miRNA223-3p.Results Expression levels of miRNA22-3p and miRNA223-3p in the observation group were(4.01±1.03)and(1.51±0.12),respectively,which were significantly higher than those in the control group[(1.12±0.26)and(0.62±0.20)](t=24.333,34.130,both P<0.05).The levels of IL-1β,TNF-αand IL-6 in the observation group were(57.77±7.24),(24.04±4.24)and(49.27±3.72),respectively,which were significantly higher than those in the control group.(42.27±3.31),(18.07±4.17),(39.75±3.73),respectively(t=17.415,8.979,16.164,all P<0.05).The levels of monocyte chemoattractant protein(MCP)-1,thymus and activation regulated chemokine(TARC),eotaxin-2 in the observation group were(106±12),(215±13),(347±11)which were higher than those in the control group[(72±11),(159±12),(317±12)](t=18.095,29.356,16.713,all P<0.05).Pearson correlation analysis showed the expressions of miRNA22-3p and miRNA223-3p were positively correlated with MCP-1,TARC,eotaxin-2,IL-1β,TNF-αand IL-6(r=0.609,0.659,0.545,0.610,0.551,0.571,0.684,0.668,0.671,0.542,0.534,0.458,all P<0.05).Conclusion The expression levels of mirNA22-3p and mirNA223-3p in gouty arthritis patients are significantly increased,which are positively correlated with inflammation and immune response.
作者
安丽欣
张倩
李昆
刘耔序
刘健
石蕊
An Lixin;Zhang Qian;Li Kun;Liu Zixu;Liu Jian;Shi Rui(The First Hospital of Qinhuangdao Rheumatology and immunology,Qinhuangdao 066000,China)
出处
《中华关节外科杂志（电子版）》
CAS
CSCD
2023年第1期29-34,共6页
Chinese Journal of Joint Surgery(Electronic Edition)
基金
秦皇岛市市级自筹项目(202101A087)
作者简介
通信作者:安丽欣,Email:mkioo110@163.com
