摘要
目的探讨高血压肾损伤患者外周血单个核细胞过氧化物酶体增殖物激活受体γ(PPARγ)和核因子-κB(NF-κB)的mRNA表达及其与心血管重构的相关性。方法选择2016年5月至2019年5月本院收治的高血压患者,65例为单纯高血压组,50例高血压肾损伤患者为高血压肾损伤组,健康者50例为对照组。测定各组受试者的外周血单个核细胞PPARγmRNA、NF-κB mRNA表达;应用心脏和血管超声分别检测颈动脉及心脏的重构指标:颈动脉平均内膜中层厚度(IMT)、颈动脉斑块积分、左心室质量指数(LVMI)。应用Pearson方法分析高血压肾损伤患者外周血单个核细胞PPARγmRNA、NF-κB mRNA表达水平与心血管重构指标的相关性,多元线性回归分析高血压肾损伤患者IMT的影响因素。结果与对照组相比,单纯高血压组、高血压肾损伤组患者的IMT、斑块积分、LVMI和NF-κB mRNA表达水平明显升高,而PPARγmRNA表达水平均明显降低(P<0.05)。高血压肾损伤组IMT、斑块积分、LVMI各指标和NF-κB mRNA表达水平均明显高于单纯高血压组,PPARγmRNA表达水平明显低于单纯高血压组(P<0.05)。高血压肾损伤患者PPARγmRNA表达水平均与IMT、斑块积分、LVMI呈负相关关系(均P<0.05);NF-κB mRNA表达水平均与IMT、斑块积分、LVMI均呈正相关关系(P均<0.05)。PPARγmRNA降低、NF-κB mRNA升高是高血压肾损伤患者IMT的影响因素(均P<0.05)。结论高血压肾损伤患者外周血PPARγmRNA表达明显降低,NF-κB mRNA表达明显升高,二者均与心血管重构有相关性。
Objective To explore the peripheral blood mononuclear cells(PBMC)expression of PPARγand NF-κB mRNA and its correlation with cardiovascular remodeling in patients with hypertensive renal injury.MethodsFrom May 2016 to May 2019 in our hospital,65 patients with hypertension were selected as the simple hypertension group,and 50 patients with hypertensive renal injury were selected as the hypertensive renal injury group,while 50 healthy persons were selected as the control group.The expression of PPARγmRNA and NF-κB mRNA in PBMC was measured.Cardiac and vascular ultrasound was used to detect the remodeling indicators of carotid artery and heart:carotid artery mean intima-media thickness(IMT),carotid plaque score,and left ventricular mass index(LVMI).Pearson analysis was used to analyze the correlation between the expression of PPARγand NF-κB mRNA and cardiovascular remodeling indexes.Multiple linear regression method was used to analyze the influencing factors of IMT in the patients with hypertensive renal injury.ResultsCompared with those in the control group,the IMT,plaque score,LVMI,and NF-κB mRNA expression were significantly higher in the simple hypertension group and hypertensive renal injury group,while the PPARγmRNA expression was significantly lower(P<0.05).The IMT,plaque score,LVMI,and NF-κB mRNA expression in the hypertensive renal injury group were significantly higher than those in the simple hypertension group,while the PPARγmRNA expression was significantly lower than that in the simple hypertension group(P<0.05).In the hypertensive renal injury group,the expression of PPARγmRNA was negatively correlated with IMT,plaque score,and LVMI(P<0.05),but the expression of NF-κB mRNA was positively correlated with IMT,plaque score,and LVMI(P<0.05).The decrease of PPARγmRNA and the increase of NF-κB mRNA were the influencing factors of IMT in patients with hypertensive renal injury(P<0.05).ConclusionThe expression of PPARγmRNA in PBMC of patients with with hypertensive renal injury was significantly decreased,while the expression of NF-κB mRNA was significantly increased.Both of them were correlated with the cardiovascular remodeling.
作者
陈光涛
吴立强
庄雄
Guangtao Chen;Liqiang Wu;Xiong Zhuang(Second Department of Internal Medicine,Hospital of Hainan Armed Police Corps,Haikou 570203,Hainan Province,China;First Department of Internal Medicine,Hospital of Hainan Armed Police Corps,Haikou 570203,Hainan Province,China;Department of Gastroenterology,Hospital of Hainan Armed Police Corps,Haikou 570203,Hainan Province,China)
出处
《中华肾病研究电子杂志》
2022年第5期270-275,共6页
Chinese Journal of Kidney Disease Investigation（Electronic Edition）
作者简介
通信作者:陈光涛,Email:qfrgh2372021@163.com
