摘要
Objective:To study the correlation of myosin light chain kinase (MLCK) and fibroblast activation protein (FAP) expression with uterine fibroid cell proliferation and invasion. Methods:Uterine fibroids samples and normal uterine muscle samples next to fibroids that were surgically removed in Wuhan Red Cross Hospital between May 2014 and January 2017 were chosen, fluorescence quantitative PCR kits were used to deterct MLCK and FAP mRNA expression, and enzyme-linked immunosorbent assay kits were used to determine proliferation and invasion gene protein expression.Results: MLCK and FAP mRNA expression in uterine fibroids samples were significantly higher than those in normal uterine muscle samples, and Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression were significantly higher than those in normal uterine muscle samples;Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression in uterine fibroids samples with high MLCK and FAP expression were significantly higher than those in uterine fibroids samples with low MLCK and FAP expression.Conclusion: Highly expressed MLCK and FAP in uterine fibroids can promote the proliferation and invasion of uterine fibroids.
作者简介
Corresponding author:Li Wang,Department of Obstetrics and Gynecology,Wuhan Red Cross Hospital(Wuhan 11th Hospital)in Hubei Province,Wuhan City,Hubei Province,430015.Tel:18062519060
