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流感病毒Real-time RT-PCR核酸检测及流行情况分析 被引量:1

Detection of Influenza Viruses Using Real-time RT-PCR and Analysis of Influenza Epidemic Situation
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摘要 目的:分析鄂尔多斯市2012年流感检测结果,为本地区流感预防控制提供科学依据。方法采用Real-time RT-PCR法对哨点医院采集的流感样病例咽拭子标本进行病毒核酸检测。结果共检测流感咽拭子标本281份,流感病毒核酸阳性35份,总阳性率为12.46%。其中乙型16份,占5.69%,季节性H3亚型15份,占5.34%,甲型H1N12份,占0.71%,甲型未分型2份,占0.71%。2012年1月~3月阳性率分别为11.36%、27.50%、37.50%,10月和12月阳性率均为5.00%,其它月份未检出阳性。结论监测显示鄂尔多斯地区流感流行季节主要为冬春季,流行毒株主要是乙型和季节性H3亚型。 Objective To analyze the results of detection on influenza virus in Ordos in 2012,in order to provide scientific basis for prevention and control of flu in this area.Methods Influenza throat swab specimens were col ected from sentinel hospital for virus nucleic acid detection by Real-time RT-PCR.Results:A total of 281 throat swab specimens were detected,35 were positive for influenza virus nucleic acid,at a percentage of 12.46%.In which there was 16 cases of B type flu,at a percentage of 5.69%,15 cases of seasonal H3,at a percentage of 5.34%,2 cases of new H1N1,at a percentage of 0.71%,2 positive of A type undentified,at a percentage of 0.71%.The detection positive rate were 11.36%、27.50%、37.50%in January,February and March,were 5.00%in October and December,no positive sample was detected in other month.Conclusion The survey showed that the major epidemic seasons were winter and spring,the major epidemic strains of the flu were B type and H3 type.
作者 冀林立 JI Lin-li(Center For Disease Control and Prevention of Erdos,Erdos 017000,Inner Mongolia,China)
出处 《医学信息(医学与计算机应用)》 2014年第10期90-91,共2页 Medical Information
关键词 流感监测 病毒核酸 Real-time RT-PCR Surveil ance of influenza Virus nucleic acid Real-time RT-PCR
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