摘要
Objective:To study the effect of temperature on the morphological characteristics of Botrytiscinerea(B.cinerea)and its correlated with the genetic variability.B.cinerea is a plant-pathogenic fungus that produces the disease known as grey mould in a wide variety of agriculturally important hosts in many countries.Methods:Six strains from different host collected have been isolated and characterized by several methods as mycelial growth,fungicide resistance,pathogenicity and the effects of the temperature.Also was analyzed by PCR and distinguished by the presence or absence of transposable elements.Results:Results showed that clear morphological differences exist between strains at the temperature of 4,12 and 28℃.All strains analyzed molecularly were classified as Group II(transposa-type).Demonstrating a negative correlation between mycelial growth and other characteristics as the fungicide resistance and pathogenicity.Lastly,it is difficult to establish relationships phenotypic and genotypic between strains of B.cinerea.Conclusions:The results indicated that the mycelial growth,resistance at fungicide and pathogenicity are independent of the characteristics molecular,however,are dependent of a factor such as temperature.
基金
Supported by National University of San Luis(PROICO 22/Q241)
the National Agency of Scientific and Technologic Promotion(PICT-2011-0459)
the National Scientific and Technical Research Council(CONICET)(PIP 112-201101-00114).
作者简介
Corresponding author:Dr.María I Sanz,Area of Chemistry and Biotechnology Technology,&INQUISAL-CONICET,Department of Chemistry,Faculty of Chemistry,Biochemistry and Pharmacy,National University of San Luis,Chacabuco 917,D5700BWS San Luis,Argentina.Tel:+54-2652-425385,Fax:+54-2652-430224,E-mail:msanz@unsl.edu.ar.
