摘要
Objective:To study the significance of mir-141 expression in COPD(chronic obstructive pulmonary diseases)mononuclear cells,and to demonstrate its effect on proliferation and apoptosis of fibroblasts Possible to ZEB1(zinc finger E-box binding homeobox 1).Methods:40 cases acute phase of COPD patients,80 cases of stable period COPD patients and 110 normal controls in our hospital were collected.RT-PCR was used to detect mir-141 and statistical analysis.The mir-141 mimic was constructed and transfected into lung fibroblasts.The expression of mir-141 was analyzed by RT-PCR.Cell proliferation was analyzed by CCK8 and cell apoptosis was detected by flow cytometry.Targetscan was used to predict the binding site of ZEB1 and mir-141.The target relationship between ZEB1 and mir-141 was identified by RT-PCR and western blot.Results:The expression of mir-141 in mononuclear cells of acute phase of COPD and stable period of COPD was significantly higher than that of normal controls.Mir-141 levels in acute phase of COPD were significantly higher than that of stable period of COPD.Mir-141 mimic transfection significantly up-regulated the expression of mir-141,promoted cell activity and decreased cell apoptosis rate compared with the empty control group.ZEB1 and mir-141 had binding sites predicted by Targetscan database and verified by dual luciferase assays.After mir-141 mimic transfection hat,ZEB1 mRNA and protein expression were down-regulated.Conclusions:High expression of mir-141 in COPD may promote the proliferation of lung fibroblasts and inhibit apoptosis by targeting ZEB1.
基金
National natural science foundation of China(81160008,81660013).
作者简介
Corresponding author:Yi-Peng Ding,Chief physician.E-mail:ypding@263.net.
