摘要
目的运用网络药理学方法探讨宁泌泰胶囊治疗慢性前列腺炎(CP)和良性前列腺增生症(BPH)的作用机制.方法通过TCMSP和BATMAN-TCM数据库获取宁泌泰胶囊的成分及作用靶点,通过Gene cards、OMIM、Dis-genet数据库获取CP和BPH的作用靶点,通过韦恩图工具获取CP和BPH的共同靶点以及宁泌泰治疗CP和BPH的有效靶点,通过STRING数据库对共有基因构建蛋白相互作用(PPI)网络,利用Cytoscape软件筛选核心靶点,构建"药物-成分-靶点"网络.利用Metascape数据库进行GO和KEGG富集分析.利用Autodock4.2.6软件对核心靶点和成分进行分子对接验证.结果筛选后得到宁泌泰胶囊有效成分47个,靶点280个,疾病靶点1445个,取交集后得到潜在治疗靶点168个,核心成分包括槲皮素、山奈酚、β-谷甾醇等,核心靶点包括AR、ESR1、TNF、IL-1B等.GO分析显示治疗靶点与对无机物质的反应、凋亡信号通路、对脂多糖的反应等过程相关,主要涉及PI3K/AKT、MAPK等信号通路.结论宁泌泰胶囊可能通过抑制促炎细胞因子的合成、抑制信号转导、调节前列腺组织细胞生长、调节雄激素水平等机制来实现"异病同治"治疗CP和BPH的作用.
Objective To investigate the mechanism of Ningmitai Capsule in the treatment of chronic prostatitis(CP)and benign prostatic hyperplasia(BPH)using network pharmacology.Methods The ingredients and action targets of Ningmitai Capsule were retrieved through TCMSP and BATMAN-TCM database,the action targets of CP and BPH were retrieved through gene cards,OMIM and Disgenet database,the common targets of CP and BPH were retrieved through Venn diagram tool and the effective targets of Ningmitai Capsule in the treatment of CP and BPH were retrieved,and the consensus gene to construct a protein interaction(PPI)network by string database,The core targets were screened using Cytoscape software to construct a"drug-component-target"network.Go and KEGG enrichment analyses were performed using the metascape database.Molecular docking validation of core targets and constituents was performed using autodock4.2.6.Results 47 active ingredients of Ningmitai Capsule with 280 targets and 1445 disease targets were obtained after screening,while 168 potential therapeutic targets were obtained after intersection,the core ingredients including quercetin,kaempferolβ-Sitosterol,among others,and core targets include AR,ESR1,TNF,IL-1B,among others.Go analysis showed that the therapeutic targets were associated with processes such as response to inorganic substances,apoptotic signaling pathway,response to lipopolysaccharide,mainly involving PI3K/Akt,MAPK and other signaling pathways.Conclusion Ningmitai Capsule may achieve the effects of"Treat Different Diseases with the Same Method"for CP and BPH by inhibiting the synthesis of proinflammatory cytokines,inhibiting signal transduction,regulating cell growth in prostate tissue,regulating androgen levels,and other mechanisms.
作者
赵丰
邓楹君
陈亚洲
高庆和
郭俊
王浩
任凯
郭军
Zhao Feng;Deng Yingjun;Chen Yazhou;Gao Qinghe;Guo Jun;Wang Hao;Ren Kai(Department of Andrology,Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing 100091,China)
出处
《中国男科学杂志》
CAS
CSCD
2022年第2期39-47,共9页
Chinese Journal of Andrology
基金
国家自然科学基金青年项目(82104880)
中国中医科学院科技创新工程(CI2021A02208)
中国中医科学院西苑医院苗圃课题项目(2019XYMP--23)
中国中医科学院西苑医院国家自然科学基金培育项目(XY20-13)
关键词
宁泌泰胶囊
异病同治
慢性前列腺炎
良性前列腺增生(症)
Ningmitai Capsule
treat different diseases with the same method
chronic prostatitis
benign prostatic hyperplasia
作者简介
通信作者:郭军,E-mail:guojunl126@126.com
