摘要
目的:探讨叉头框蛋白O1(FoxO1)介导血管内皮生长因子(VEGFA)对大鼠骨折愈合及软骨新生血管的影响。方法:SD大鼠30只随机分为假手术组、骨折组、干预组,每组10只,骨折组和干预组制备右侧股骨中段骨折模型,待各组大鼠完全清醒后,干预组尾静脉注射溶于15 ml林格氏液的200μg pcDNA3.1-FoxO1重组质粒,假手术组和骨折组尾静脉注射等量生理盐水,16 d后,比较各组大鼠股骨密度、骨折区骨痂面积及骨痂组织中FoxO1、VEGFA、新生血管形成标志物CD31蛋白表达量。结果:干预组和骨折组大鼠软骨组织中FoxO1、VEGFA、CD31蛋白表达量显著高于假手术组(P<0.05);干预组大鼠软骨组织中FoxO1、VEGFA、CD31蛋白表达量显著高于骨折组(P<0.05);干预组大鼠骨折区骨痂面积显著高于骨折组,差异具有统计学意义[(0.23±0.05)cm^2比(0.12±0.02)cm^2,P<0.05];各组大鼠股骨密度差异无统计学意义(P>0.05)。结论:FoxO1可能通过上调VEGFA而促进软骨血管内皮细胞形成血管,从而促进骨折愈合。
Objective:To investigate the effect of vascular endothelial growth factor A(VEGFA)mediated by transcription factor forkhead box O1(FoxO1)on fracture healing and cartilage angiogenesis in rats.Methods:Thirty SD rats were randomly divided into sham operation group,fracture group and intervention group(n=10 each).The rats in the fracture group and intervention group were modeled for right midshaft femur fracture.After fully awaken,the rats in the intervention group were injected with 200μg recombinant plasmid pcDNA3.1-FoxO1 dissolved in 15 ml Ringer’s solution via caudal vein,while those in the sham operation group and fracture group were injected with same amount of normal saline.At 16 days later,the femoral bone mineral density,callus area in fracture area and expression levels of FoxO1,VEGFA and CD31 proteins in callus were compared among groups.Results:The expression levels of FoxO1,VEGFA and CD31 proteins in cartilage tissue was the significantly higher in the intervention group and fracture group than those in the sham operation group,and also higher in the intervention group than those in the fracture group(P<0.05).The area of bone callus was significantly larger in the intervention group than that in the fracture group,with statistically significant difference[(0.23±0.05)cm^2 vs(0.12±0.02)cm^2,P<0.05].The femoral bone mineral density did not differ significantly among three groups(P>0.05).Conclusion:FoxO1 may promote fracture healing by upregulating VEGFA to promote cartilage angiogenesis.
作者
顾光学
霍永锋
殷照阳
徐刚
Gu Guangxue;Huo Yongfeng;Yin Zhaoyang;Xu Gang(Department of Traumatology and Orthopedics,Lianyungang First People’s Hospital,Lianyungang 222000,Jiangsu,China)
出处
《中华生物医学工程杂志》
CAS
2020年第4期319-322,共4页
Chinese Journal of Biomedical Engineering
作者简介
通信作者:顾光学,Email:chenjing81130i@sina.com
