摘要
为筛选茶炭疽病病原(C.camelliae)高效化学农药。采用菌丝速率生长法,测试14种农药在100μg/mL浓度下的抑菌活性及其EC50。结果发现,14种农药中,咪鲜胺、氟硅唑、甲基硫菌灵、苯醚甲环唑、肟菌·戊唑醇和氟啶胺对茶炭疽病的抑菌活性均超过90%;EC50以氟啶胺(0.14μg/mL)、咪鲜胺(0.59μg/mL)和氟硅唑(1.78μg/mL)较优,分别比阳性对照萎锈灵(106.24μg/mL)减少用药量106.1μg/mL、105.65μg/mL和104.46μg/mL。随着氟啶胺、咪鲜胺和氟硅唑浓度上升,受试菌丝从光滑、均匀的形态变为严重畸形,并出现较多分支和不规整瘤状突起。氟啶胺、咪鲜胺和氟硅唑具有较好的离体抑制活性,可能是潜在的抗茶炭疽病病原(C.camelliae)高效药剂,但尚需田间药效试验进行验证。
Screening of efficient chemical pesticides against tea anthracnose pathogen C.camelliae.The bacteriostatic activity and EC50 of 14 pesticides at a concentration of 100μg/mL were tested by the mycelial growth method.The results showed that among the 14 kinds of pesti-cides,the antibacterial activities of prochloraz,flusilazole,thiophanate-methyl,difenoconazole,oxime tebuconazole and fluazinam on tea an-thracnose all exceeded 90%;and EC50 of fluazinam(0.14μg/mL),prochloraz(0.59μg/mL)and flusilazole(1.78μg/mL)were better,and the dosage(106.1μg/mL,105.65μg/mL and 104.46μg/mL)was lower than that of the positive control,chlorpyrifos(106.24μg/mL),respectively.As the concentrations of fluazinam,prochloraz and flusilazole increased,the tested hyphae changed from smooth and uniform mor-phology to severely deformed,with more branches and irregular tumor-like protrusions.Fluazinam,prochloraz and flusilazole have good inhibi-tory activities in vitro,and may be potential high-efficiency agents against tea anthracnose pathogen C.camelliae,but field efficacy tests are needed to verify them.
作者
饶家瑞
孟泽洪
李帅
张金峰
周玉锋
RAO Jia-rui;MENG Ze-hong;LI Shuai;ZHANG Jin-feng;ZHOU Yu-feng(Guizhou Tea Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang Guizhou 550006,China)
出处
《贵茶》
2022年第3期37-43,共7页
Journal of Guizhou Tea
基金
黔农科院青年基金[2022]29号
财政部和农业农村部
国家现代农业产业技术体系资助(CARS-19)
关键词
茶炭疽病
山茶刺孢菌
氟啶胺
咪鲜胺
氟硅唑
Tea Anthracnose
Colletotrichum camelliae
Fluazinam
Prochloraz
Flusilazole
作者简介
饶家瑞(1992-),男,贵州贵阳人,硕士,研究实习员,主要从事茶树病害绿色防控研究。E-mail:847532347@qq.com;通讯作者:周玉锋(1971-),男,贵州道真人,博士,研究员,主要从事茶树病害绿色防控研究。E-mail:gzzhouyf@163.com.
