摘要
目的本研究旨在探究糖尿病肾病患者和健康体检者血液样本中外泌体hsa-miR-377的表达量,以表明其参与糖尿病肾病的致病过程中。方法本研究通过收集临床糖尿病肾病患者和健康体检者的血液样本进行外泌体分离鉴定,通过透射电子显微镜对血液样本获得的离心小球进行分析,再进行RT-PCR分析外泌体中小RNA分子的表达。结果TEM发现糖尿病肾病的血液样本中存在外泌体,且外泌体形态在镜下很明显,RT-PCR对外泌体中小RNA分子的表达结果显示外泌体中hsa-miR-377的表达量(0.65±0.05)普遍高于正常体检者血液样品(0.29±0.05)(P<0.01)。结论外泌体hsa-miR-377可能参与糖尿病肾病的致病过程,且可能是糖尿病肾病的一个潜在诊断标志物。
Objective In this subject,we aim to explore the expression of the exosome hsa-miR-377 in the blood samples of the diabetic nephropathy patients and healthy controls,which make us get to know that the exosome hsa-miR-377 plays important roles in the pathology process of diabetic nephropathy.Methods Firstly,we collected the blood samples of the diabetic nephropathy patients and healthy controls,then centrifuged the blood samples with high speed density gradient and detected it with transmission electron microscopy.Lastly,we analyzed the expression of the exosome hsa-miR-377 withreverse transcription-polymerase chain reaction methods.Results It showed that the exosome is evidently exist in the bloodsamples of the diabetic nephropathy patients and looks like a branched spheroid outlook.Additionally,there was significant difference about the expression of the exosome hsa-miR-377 in the blood samples between the diabetic nephropathy patients(0.65±0.05)and healthy controls(0.29±0.05)(P<0.01).Conclusion There is great possibility that the exosome hsa-miR-377 plays important roles in the pathology process of diabetic nephropathy and it has potential to be a diagnostic biomarker in diabetic nephropathy.
作者
王刚
刘涛
WANG Gang;LIU Tao(Nephrology,Wuhan Hospital of Traditional Chinese Medicine)
出处
《解剖学研究》
CAS
2020年第3期241-243,共3页
Anatomy Research
基金
武汉市卫生和计划生育委员会科研项目(WZ18D21)
作者简介
通信作者:刘涛,E⁃mail:gangqianchi@163.com
