摘要
采收是规模化生产微藻过程中的重要环节,其技术是目前制约微藻产业化发展水平的关键因素之一。本研究以明矾为絮凝剂研究了明矾浓度、初始OD值和絮凝时间对小球藻(Chlorella sorokiniana)絮凝效率的影响,并在此基础上进一步采用响应面法优化了明矾絮凝采收小球藻的工艺条件,为规模化絮凝采收小球藻提供了技术支持和数据参考。研究结果如下:(1)明矾浓度、初始OD值和絮凝时间均对小球藻絮凝效率有显著性影响(P<0.05),影响小球藻絮凝效率的主次顺序是明矾浓度>初始OD值>絮凝时间;(2)利用明矾絮凝采收小球藻的最佳工艺条件为:明矾浓度1.2 g/L、初始OD值0.8、絮凝时间30 min,在此条件下小球藻的絮凝效率可达到98.49%。研究结果将为规模化应用明矾絮凝采收小球藻的工艺提供数据参考及技术支持。
During the large-scale production of microalgae,harvesting technology is one of the important steps,and is one of the key factors currently restricting the algal industrialized development.In this study,alum was used as a flocculant to investigate the effects of alum concentrations,initial OD values,and flocculation time on the flocculation efficiency of Chlorella sorokiniana.And on this basis,response surface methodology was applied to optimize the flocculation process of C.sorokiniana using alum.It was hoped that these results would provide technical support and data reference for large-scale harvest of this alga.Main experimental results were as follows:(1)alum concentrations,initial OD values,and flocculation time had significant effects on the flocculation efficiency of C.sorokiniana(P<0.05),the factors that affected the flocculation efficiency were in the order of alum concentrations>initial OD values>flocculation time;(2)the highest flocculation efficiency of C.sorokiniana(98.48%)was obtained under the optimal technological conditions for flocculation process of this alga,which were 1.2 g/L of alum,0.8 of initial OD value,and 30 min of flocculation time.
作者
薛春叶
陈彪
曹倍
董京伟
颜闰娇
邓祥元
Xue Chunye;Chen Biao;Cao Bei;Dong Jingwei;Yan Runjiao;Deng Xiangyuan(College of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang,212018)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第7期2793-2800,共8页
Genomics and Applied Biology
基金
江苏省第十五批“六大人才高峰”高层次人才选拔培养项目(SWYY-025)
江苏省镇江市重点研发计划(社会发展)项目(SH2019004)共同资助
关键词
明矾
小球藻
絮凝
响应面法
Alum
Chlorella sorokiniana
Flocculation
Response surface methodology
作者简介
通信作者:邓祥元,dengxy2009@126.com
