摘要
人Ⅱ型肝脏表达抗菌肽(LEAP-2)作为新型活性多肽,具有抑菌活性和调节体重的功能。为了深入认识人LEAP-2的基本信息,本研究利用在线生物软件和工具进行了全面系统的生物信息学分析。一级结构分析结果表明,人LEAP-2含有77个氨基酸残基,包括18种氨基酸,分子量为8.81 kDa,理论等电点为10.31,为不稳定亲水性蛋白,由信号肽、前体肽和成熟肽三部分组成,有4个糖基化和7个磷酸化修饰位点。二级结构主要元件为α螺旋和无规则卷曲。成熟肽三维结构由N端疏水柔性区、中央刚性核心区和C端柔性区组成,两对二硫键稳定空间构象。多序列比对结果表明,不同物种LEAP-2的信号肽和前体肽序列差异较大,但成熟肽序列具有极高的相似度,在长期进化过程中高度保守。系统进化树分析表明,在不同物种中LEAP-2的分子进化过程与物种亲缘进化关系基本吻合。研究结果为LEAP-2生物学功能及其分子机制的深入研究提供了理论基础。
Human liver-expressed antimicrobial peptide 2(LEAP-2)as a novel active peptide exhibits antimicrobial activities and regulates body weight.To understand the basic information of human LEAP-2,in this study,we utilized online biological software and tools for systematic bioinformatics analysis.The analysis of the primary structure showed that human LEAP-2 had 77 amino acid residues,consisting of 18 kinds of amino acids.Molecular weight was 8.81 kDa,and theoretical pI was 10.31.Human LEAP-2 presented an instable hydrophilic protein,composed of signal peptide,propeptide and mature peptide.There were 4 glycosylation sites and 7 phosphorylation sites.The main secondary structural elements wereα-helix and random coil.Three-dimensional structure of the mature peptide was composed of hydrophobic flexible N terminus,rigid central core and flexible C terminus,stabilized by the two disulfide bonds.The results of multiple sequence alignment of LEAP-2 from different species revealed that the sequences of signal peptide and propeptide had a certain difference,while the mature peptide sequences displayed high similarity and kept highly conserved region during long evolutionary process.The analysis of phylogenetic tree suggested that molecular evolution process of LEAP-2 in different species was basically consistent with phylogenetic relationship of species.The research results provide a theoretical basis for further in-depth study of the biological function and molecular mechanism of LEAP-2.
作者
王珊珊
高琪
任艳艳
路宏朝
王令
张涛
Wang Shanshan;Gao Qi;Ren Yanyan;Lu Hongzhao;Wang Ling;Zhang Tao(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,723001)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2022年第2期407-414,共8页
Genomics and Applied Biology
基金
陕西省教育厅重点科学研究计划项目(20JY006)
陕西省教育厅专项科研计划项目(20JK0574)
陕西省科技厅重点研发计划项目(2017NY-162)
陕西理工大学科研基金项目(SLGQD16-05)共同资助
关键词
人LEAP-2
生物信息学分析
成熟肽
三维结构
多序列比对
Human LEAP-2
Bioinformatics analysis
Mature peptide
Three-dimensional structure
Multiple sequence alignment
作者简介
通信作者:张涛,zhangtao780823@snut.edu.cn
