摘要
磷酸奥司他韦(oseltamivir phosphate,OP)是治疗甲型和乙型病毒性感冒的特效药。本文通过宏基因组测序、气相色谱和高效液相色谱技术,研究磷酸奥司他韦对人体肠道微生物群落的体外影响,为磷酸奥司他韦的临床应用提供肠道菌群方面的数据支持。研究结果显示,磷酸奥司他韦显著降低拟杆菌科(Bacteroidaceae)、丙酸蛛网菌(Arachnia propionica)、子囊菌门(Ascomycota)和微小噬菌体科(Microviridae)的相对丰度,显著提高多动物链球菌(Streptococcus pluranimalium)、苏黎世克罗诺杆菌(Cronobacter turicensis)和担子菌门(Basidiomycota)的相对丰度(P<0.05)。另外,磷酸奥司他韦对新月形单胞菌科(Selenomonadaceae)、乳酸菌科(Lactobacillaceae)、普雷沃菌科(Prevotellaceae)、单形巨单胞菌(Megamonas funiformis)、黏膜乳杆菌(Limosilactobacillus mucosae)、粪源普雷沃氏菌(Prevotella copri)和青春双歧杆菌(Bifidobacterium adolescentis)的相对丰度有一定影响,对代谢通路和短链脂肪酸(short-chain fatty acids,SCFAs)的影响不明显。在功能基因方面,磷酸奥司他韦仅显著影响异柠檬酸脱氢酶、D-氨酰-tRNA脱酰基酶和7-羧基-7-去氮嘌呤合酶的基因丰度(P<0.05)。肠道菌群对磷酸奥司他韦降解的个体性差异较大,各组磷酸奥司他韦的平均降解率在8.6%~88.4%。总之,磷酸奥司他韦在体外发酵过程中对人体肠道菌群结构、代谢通路及其代谢产物等的影响较小。
Oseltamivir phosphate(OP)is a specific drug for the treatment of influenza A and B.In this study,the effect of oseltamivir phosphate on human gut microbiota in vitro was investigated by using metagenome sequencing,gas chromatography and high-performance liquid chromatography,to provide data for the clinical application of oseltamivir phosphate from the perspective of gut microbiota.The results indicated that oseltamivir phosphate significantly reduced the relative abundance of Bacteroidaceae,Arachnia propionica,Ascomycota and Microviridae,and significantly increased the relative abundance of Streptococcus pluranimalium,Cronobacter turicensis and Basidiomycota(P<0.05).Additionally,oseltamivir phosphate slightly affected the relative abundance of Selenomonadaceae,Lactobacillaceae,Prevotellaceae,Megamonas funiformis,Limosilactobacillus mucosae,Prevotella copri,Bifidobacterium adolescentis,and the metabolic pathways and short-chain fatty acids(SCFAs)production.At the functional gene level,oseltamivir phosphate significantly affected the gene abundance of isocitrate dehydrogenase,D-aminoacyl-tRNA deacylase,and 7-carboxy-7-deazaguanine synthase(P<0.05).The degradation of oseltamivir phosphate by gut microbiota was different among the samples,and the average degradation rate was between 8.6%and 88.4%among different groups.In summary,oseltamivir phosphate has little effect on the human gut bacterial community,their metabolic pathway and metabolites during in vitro fermentation.
作者
赵昌会
陈华海
胡云霏
李百元
曹林艳
蒋琼凤
尹业师
Zhao Changhui;Chen Huahai;Hu Yunfei;Li Baiyuan;Cao Linyan;Jiang Qiongfeng;Yin Yeshi(Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South,Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling Area,College of Chemistry and Bioengineering,Hunan University of Science and Engineering,Yongzhou,425199)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2022年第11期2120-2129,共10页
Genomics and Applied Biology
基金
国家自然科学基金区域创新发展联合基金重点支持项目(U21A20411)
湖南省自然科学基金项目(2020JJ2016)共同资助
关键词
人体肠道菌群
磷酸奥司他韦
宏基因组测序
短链脂肪酸
高效液相色谱
Human gut microbiota
Oseltamivir phosphate
Metagenome sequencing
Short-chain fatty acids
High performance liquid chromatography
作者简介
通信作者:尹业师,yinyeshi@126.com
