摘要
吸氧对运动疲劳消除和体能恢复的影响和机理尚不完全清楚。本研究从血清代谢组学角度,探讨高强度运动后常压高氧吸入对运动疲劳恢复的影响。招募有运动习惯且体能良好的军校大学生,随机分为对照组与吸氧恢复组,以自行车功率测试负荷递增的方法达到力竭。吸氧组在运动后恢复期给予常压高氧吸入(4 L/min,40 min),对照组被动恢复。检测运动前、运动结束和恢复期的血压、心率、心率变异性、反应时等生理指标。恢复60 min后外周静脉取血,进行血清生化指标和代谢组学检测。结果:1)与对照组相比,吸氧恢复组血清样本中筛选出91种差异代谢物,其中,尿酸、肌酸酐、谷氨酸显著降低,色氨酸、缬氨酸、β-丙氨酸水平显著升高。2)吸氧恢复组血清差异代谢物在赖氨酸降解、谷胱甘肽代谢、5-羟色胺能合成、支链氨基酸代谢、神经活性配受体互作、氨基糖核糖代谢、中心碳代谢等代谢途径富集。3)吸氧恢复后血清氧化应激相关代谢物降低,生化检测证实吸氧可减轻机体运动后的氧化应激相关分子8-羟基脱氧鸟苷和丙二醛;吸氧恢复后血清肾上腺素显著降低,且心率变异性结果显示时域指标窦性心搏RR间期的标准差(SDNN)显著升高,频域指标低频/高频(LF/HF)显著降低,提示吸氧降低交感神经活性,促进运动疲劳时交感-迷走神经活性平衡的恢复。综上,常压高氧吸入可改善机体代谢、减轻氧化应激和肾上腺素水平,改善心率变异性,提示吸氧有助于促进运动疲劳消除。
The effects and underlying mechanism of normobaric hyperoxia on the recovery from exercise fatigue are still not fully understood.Therefore,this study aims to investigate the effects of oxygen inhalation at atmospheric pressure on exercise fatigue following high intensity exercise through serum metabolomics changes.College students with regular exercise and good physical fitness were recruited and randomly divided into a control group(Ctrl)and an oxygen inhalation recovery group(O2).All participants underwent exhaustive exercise test on cycle ergometer.The control group underwent passive recovery,while the oxygen inhalation group was given normobaric hyperoxia treatment(4 L/min,40 min).Physiological indicators such as blood pressure,heart rate,heart rate variability(HRV),and reaction time were measured before exercise,immediately after exercise and during the recovery period.After 60 minutes of recovery,blood samples were collected from peripheral veins for serum biochemical markers and metabolomics analysis.Results:1)Compared to the control group,there were 91 differential serum metabolites in oxygen inhalation recovery group,among which the levels of uric acid,creatinine and glutamate were significantly decreased,while the levels of tryptophan,valine and β-alanine were significantly increased.2)The serum differential metabolites resulted in the enrichment of lysine degradation,glutathione metabolism,5-hydroxytryptamineic synthesis,branched-chain amino acid metabolism,neuroactive ligand-receptor interaction,amino sugar and nucleotide sugar metabolism,central carbon metabolism and other metabolic pathways in the oxygen inhalation recovery group.3)After oxygen inhalation recovery,the serum levels of oxidative stress-related metabolites were decreased.Biochemical analysis confirmed that oxygen inhalation can alleviate postexercise oxidative stress-related molecules,such as 8-OHdG and MDA.Additionally,the serum adrenaline was significantly decreased after oxygen inhalation recovery.The HRV data showed a significant increase in the standard deviation of the RR interval(SDNN)and a significant decrease in the LF/HF index,suggesting that oxygen inhalation reduced the sympathetic activity and promoted the restoration of the sympathetic-parasympathetic imbalance caused by exercise fatigue.In conclusion,normobaric hyperoxia enhances metabolism,reduces oxidative stress and adrenaline,and promote recovery of HRV,suggesting that oxygen inhalation helps promote the elimination of exercise fatigue.
作者
邢文娟
董玲
周嘉恒
张伟
胡扬
张鹏飞
高炳宏
高峰
XING Wenjuan;DONG Ling;ZHOU Jiaheng;ZHANG Wei;HU Yang;ZHANG Pengfei;GAO Binghong;GAO Feng(Aerospace Medicine School,Air Force Medical University,Xi’an 710032,Shaanxi China;School of Athletic Performance,Shanghai University of Sport,Shanghai 200438,China)
出处
《北京体育大学学报》
CSSCI
北大核心
2023年第7期28-41,共14页
Journal of Beijing Sport University
基金
国家重点研发计划“科技冬奥”重点专项“冬季项目的体能训练与训练监控关键技术”(项目编号:2019YFF0301600)
陕西省青年科技新星项目(项目编号:2021kjxx21)。
关键词
常压高氧
运动疲劳
代谢组学
氧化应激
心率变异性
normobaric hyperoxia
exercise fatigue
metabolomics
oxidative stress
heart rate variability
作者简介
邢文娟,副教授,博士,硕士研究生导师,研究方向运动与心血管保护。
