摘要
钛本身为生物惰性材料,为了提高钛植入体的生物活性,制备出兼具微米级结构和纳米级结构的表面,发挥微纳米双级结构的协同效应。采用纳秒激光刻蚀出微沟槽结构并在其表面进行阳极氧化,在钛表面制备了一种有序的微沟槽-Ti O2纳米管复合结构。对各组不同表面结构的试件的表面形貌、粗糙度、亲水性、物相组成等进行表征。应用生物矿化试验对不同组试件的生物活性进行评价。与抛光表面相比,微纳米复合结构的表面粗糙度从0.281μm增加到7.297μm,表面接触角从73.1°减小到32.1°,亲水性显著提高。XRD图谱显示,阳极氧化后经热处理的表面出现了锐钛矿(2θ=26°)的特征峰,表明Ti O2由无定型转变为锐钛矿型。此外,与抛光表面和单一微/纳米结构表面相比,微纳组表面在模拟体液中浸泡14 d后沉积的羟基磷灰石层更致密。采用激光刻蚀与阳极氧化制备的微纳复合结构可以显著提高钛表面的粗糙度和亲水性,且具有更加优异的生物活性。此研究为在钛植入体表面构建规则的微纳米结构以改善生物活性提供了一种有效的方法。
Titanium and its alloys are bioinert materials.The surface with micro-scale and nano-scale structures was prepared and the synergistic effect of the micro-nano structure was used to improve the biological activity of the titanium implant.An ordered micro-grooves and Ti O2 nanotube composite structure was prepared on titanium by nanosecond laser etching and anodic oxidation.The surface morphology,roughness,hydrophilicity,and phase composition of Ti samples with different surface structures were characterized.Vitro mineralization experiments were used to evaluate the biological activity of samples with different groups.Results show that micro-grooves and Ti O2 nanotubes are successfully constructed.Compared with polished surface,the surface roughness of the micro-nano composite structure increase from 0.281μm to 7.297μm,and the surface contact angle decrease from 73.1°to 32.1°,exhibiting a better hydrophilicity.XRD patterns show that the anatase characteristic peak appeares after anodization and heat treatment,indicating that Ti O2 changed from amorphous to anatase.In addition,after being immersed in the simulated body fluid for 14 days,the hydroxyapatite layer deposited on the micro-nano structured surface is thicker and denser,compared with the polished surface and the single micro/nano structured surface.All results indicated that micro-nano structure prepared by laser etching and anodic oxidation can significantly improve the roughness and hydrophilicity of the titanium surface,and realized more excellent biological activity.This study provides an effective method for constructing regular micro-nano structures on the surface of titanium implants to improve biological activity.
作者
赵梓贺
万熠
于明志
张晓
王宏卫
宋章仪
ZHAO Zihe;WAN Yi;YU Mingzhi;ZHANG Xiao;WANG Hongwei;SONG Zhangyi(Key Laboratory of Ministry of Education for High-efficiency and Clean Mechanical Manufacture,Shandong University,Jinan 250061,China;School of Mechanical Engineering,Shandong University,Jinan 250061,China)
出处
《中国表面工程》
EI
CAS
CSCD
北大核心
2020年第6期29-36,共8页
China Surface Engineering
基金
国家自然科学基金(51975336)
山东省重点研发计划(2019JZZY010112)
山东省自然科学基金重大基础研究项目(ZR2018ZB0106)
关键词
钛植入体
激光刻蚀
阳极氧化
微纳米结构
羟基磷灰石
生物活性
titanium implant
laser etching
anodization
micro-nano structure
hydroxyapatite
biological activity
作者简介
通信作者:万熠(1977—),男(汉),教授,博士,研究方向:生物材料加工制造理论与技术,E-mail:wanyi@sdu.edu.cn
