摘要
本文以玉米品种——御喜198为材料,用培养皿加滤纸培养法与震荡浸种的方法,研究了不同浓度纳米二氧化锆对种子萌发及代谢相关酶活性的影响.研究结果表明:在纳米二氧化锆浓度为10~150 mg·L^(-1)时,对玉米种子的萌发和根、芽的生长有明显的促进作用;纳米二氧化锆浓度为100 mg·L^(-1)时,玉米种子的芽长、芽重、根重分别达到最大值;在浓度为130 mg·L^(-1)时玉米种子的根长达到最大值;但是当纳米二氧化锆的浓度大于600 mg·L^(-1)时,试验组的结果相较于对照组有所降低,开始出现抑制生长的现象.此外,二氧化锆(130 mg·L^(-1))可以显著增加种子萌发过程糖酵解途径中关键酶己糖激酶和磷酸果糖激酶活性.因此,玉米种子在一定浓度纳米二氧化锆的处理下,通过增强糖酵解代谢活性促进种子的萌发.
In order to understand the effect of nano-zirconium dioxide on the germination of maize seeds,the method of petri dish using filter and shake immersion was adopted to explore the effect of different nanozirconium dioxide concentration on seed germination and metabolism related enzymes activity in maize(maize varieties-yuxi 198).The results showed that nano-zirconium dioxide had a significant improving effect on germination rate,the growth of maize seed root and bud,when the concentration of nano-zirconium dioxide was from 10 to 150 mg·L^(-1).Especially,when its concentration was 100 mg·L^(-1),compared with the control sample,the bud length,bud weight and root weight number of maize seeds reached the maximum value,respectively.The root length of corn seeds reached the maximum value at 130 mg·L^(-1)nano-Zirconium dioxide.However,these test data of maize seeds were lower than the control sample when the concentration of nano-zirconium dioxide was over 600 mg·L^(-1).What is more,the nano-zirconium dioxide(130 mg·L^(-1))could significantly increase hexokinase and fructose phosphate kinase activity of maize seeds which as key enzyme from glycolytic pathway.Therefore,nano-zirconium dioxide with the appropriate concentration could promote germination rate through enhanced glycolysis metabolic activity.
作者
罗志勇
熊睿鹏
袁振忠
周倩
Luo Zhiyong;Xiong Ruipeng;Yuan Zhenzhong;Zhou Qian(School of Chemistry and Environmental Science,Xiangnan University,Chenzhou 423000,China)
出处
《湘南学院学报》
2022年第2期120-125,共6页
Journal of Xiangnan University
基金
湖南省教育厅科学研究项目(19C1705)
关键词
纳米二氧化锆
萌发率
酶活性
玉米
nano-zirconium dioxide
germination rate
enzyme activity
maize
作者简介
罗志勇(2000—),男,湖南长沙人,本科生,研究方向:生物技术;通信作者:周倩(1993—),女,湖南郴州人,助理实验师,硕士,研究方向:作物营养与施肥.
