摘要
为获得能有效抑制烟草赤星病病菌的生防拮抗菌,采用平板对峙法进行菌株筛选,再用菌丝生长速率法和离体叶片试验法对拮抗菌的拮抗效果进行测试,然后通过形态、生理生化特征以及16S rDNA、gyrA序列分析等鉴定拮抗菌种类,并对其生长条件进行了优化。结果筛选出一株有一定抑菌效果的拮抗菌T11,其平板抑菌率达到45.9%,离体烟叶试验法平均防效为64.6%,无菌滤液对菌丝生长的抑制率达到60.6%;经鉴定T11菌株为枯草芽孢杆菌(Bacillus subtilis),最适生长的营养条件组合为牛肉膏5 g/L、蔗糖15 g/L、MgSO4·7H_(2)O15 g/L,该菌株及相关营养条件可为烟草赤星病生物防治提供参考。
In order to obtain antagonistic bacteria which can effectively inhibit Alternaria alternata.Plate confrontation method was used for screening,and mycelial rate method and in vitro leaf test were used to test the antagonistic effect of isolated strains.The antagonistic bacteria species were identified by morphological,physiological and biochemical characteristics,16 S rDNA and gyrA sequence analysis.Then,their growth media were optimized.An antagonistic strain T11 with certain inhibition to the mycelial growth was screened out.The plate inhibition rate was 45.9%,the average control effect of tobacco leaf in vitro test was 64.6%,and the inhibition rate on mycelial growth was 60.6%.The strain T11 was identified as B.subtilis.The optimized medium for the strain T11 was beef extract 5 g/L,sucrose 15 g/L,MgSO_(4)·7H_(2)O 15 g/L.It provides a new reference for biological control of tobacco brown spot.
作者
杨德政
孙光军
桑维钧
YANG De-zheng;SUN Guang-jun;SANG Wei-jun(College of Tobacco Science,Guizhou University,Guiyang 550025,China;Tobacco Department Guizhou Branch of China Tobacco Corporation,Guiyang 550025,China)
出处
《湖北农业科学》
2021年第S01期173-177,共5页
Hubei Agricultural Sciences
基金
中国烟草总公司贵州省公司项目(黔烟科合201920)
关键词
烟草赤星病
生防防治
拮抗菌
筛选鉴定
生长条件
tobacco brown spot disease
biocontrol
antagonistic bacteria
screening and identification
growth conditions
作者简介
杨德政(1995-),男,贵州凯里人,在读硕士研究生,研究方向为烟草病害防治,(电话)13985822289(电子信箱)469344083@qq.com;通信作者:桑维钧(1963-),男,贵州赤水人,教授,主要从事植物病理学研究,(电子信箱)984139246@qq.com
