摘要
从山西石油重度污染的土壤中利用筛选培养基分离、筛选获得1株二苯并噻吩(Dibenzothiophene,DBT)降解菌。利用16S rDNA序列分析对该菌株进行鉴定,结果表明:菌株ZYL-1鉴定为红球菌(Rhodococcus sp.),以红球菌ZYL-1对DBT的降解率为响应值,采用单因素和响应面法优化红球菌ZYL-1降解DBT的条件。单因素试验得到4个影响DBT降解的主要因素为底物浓度、pH、接种量和温度。响应面法优化ZYL-1对DBT的最优降解条件为底物浓度5.0 mg/L,pH 7.1,接种量6.8%,温度为34.4℃时,DBT降解率最高,为87.3%。研究为制备高效二苯并噻吩降解菌剂提供优质菌株资源,对修复石油污染土壤具有重要意义。
In this study,a dibenzothiophene(DBT)degrading strain was isolated and screened from the oil contaminated soil in Shanxi Province.The strain was identified by 16S rDNA gene sequence analysis.The results showed that strain ZYL-1 was identified as Rhodococcus sp.Single factor and response surface methodology were used to optimize the degradation conditions of DBT by Rhodococcus ZYL-1 using degradation rate of DBT as response value.The single factor experiment showed that the four main factors affecting DBT degradation were substrate concentration,pH,inoculation amount and temperature.The optimal degradation conditions of DBT by ZYL-1 optimized by response surface methodology were as follows:substrate concentration 5.0 mg/L,pH 7.1,inoculation amount 6.8%,temperature 34.4℃,the highest degradation rate of DBT was 87.3%.This study provided high-quality bacterial resources for the preparation of efficient dibenzothiophene degrading agent,which was of great significance for the remediation of oil-contaminated soil.
作者
左一琳
张奎
刘蕊
丁晓艳
方昭
李季
魏雨泉
Zuo Yilin;Zhang Kui;Liu Rui;Ding Xiaoyan;Fang Zhao;Li Ji;Wei Yuquan(College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193,China;Organic Recycling Research Institute(Suzhou),China Agricultural University,Suzhou 215100,China)
出处
《环境工程》
CAS
CSCD
北大核心
2023年第S02期712-718,共7页
Environmental Engineering
基金
国家重点研发计划(2020YFC1807901)
国家自然科学基金(42007031)
关键词
二苯并噻吩
响应面法
生物降解
石油降解菌
dibenzothiophene
response surface methodology
biodegradation
petroleum-degrading bacteria
作者简介
第一作者:左一琳(1997-),女,硕士研究生,主要研究方向为场地微生物修复。yilin_zuo@163.com;通信作者:魏雨泉(1991-),男,博士后,副教授,主要研究方向为生态工程与有机废弃物处理。weiyq2019@cau.edu.cn
