摘要
采集石河子市供暖时期的PM2.5样品,使用ICP-MS对PM2.5中5种重金属元素(Ni、Cu、Zn、Pb、Fe)进行检测,并对重金属元素进行健康风险评价.选取重金属元素中的Zn进行动物实验,以观察PM2.5及其Zn元素对大鼠肺部的影响.将不同浓度的PM2.5(0.5、3.0、15.0 mg·kg-1体重)和Zn(0.06、0.3、1.5 mg·kg-1体重)通过气管滴注的方式暴露于大鼠来探究PM2.5中的Zn元素对大鼠肺部的损伤.处死大鼠后获取大鼠的支气管肺泡灌洗液(BALF)和肺组织,使用ELISA、比色法和HE染色来检测大鼠BALF中促炎因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、肺组织中氧化应激指标超氧化物歧化酶(SOD)、丙二醛(MDA)以及观察大鼠肺组织的病理变化.结果表明供暖期间石河子市PM2.5浓度为109.85±58.76μg·m-3,PM2.5中重金属的浓度为Fe (27.766μg·m-3)> Zn (3.484μg·m-3)> Pb (1.444μg·m-3)> Cu (0.628μg·m-3)> Ni (0.094μg·m-3).Ni存在一定的致癌风险,Cu,Zn,Pb不存在非致癌风险.PM2.5以及Zn浓度的升高能够显著抑制SOD活性并增加MDA、IL-6、TNF-α水平,但是在同等Zn元素含量的情况下(PM2.5高剂量组和Zn中剂量组),PM2.5对大鼠的损伤更为严重.因此,PM2.5及其Zn元素可以引起大鼠肺部的损伤,在这一过程中Zn元素发挥重要作用.
PM2.5 samples were collected during the heating period in Shihezi City. Five heavy metal elements(Ni, Cu, Zn, Pb, Fe) in PM2.5 were detected by ICP-MS, and the health risk of heavy metal elements were evaluated. Zn was selected for animal experiments to observe the effects of PM2.5 and its Zn on lungs in rats. Different concentrations of PM2.5(0.5, 3.0 and 15.0 mg·kg-1 body weight) and Zn(0.06, 0.3 and 1.5 mg·kg-1 body weight) were exposed to rats by tracheal instillation to investigate the damage of Zn in PM2.5 to the lungs of rats. Rats were sacrificed, and the bronchoalveolar lavage fluid(BALF) and lung tissue were obtained. ELISA, colorimetry and HE staining were used to detect pro-Finflammatory factors(interleukin-F6(IL-F6), tumor necrosis factor-Fα(TNF-Fα)) in rat BALF, oxidative stress indicators(superoxide dismutase(SOD), malondialdehyde(MDA)) and pathological changes in rat lung tissues. The results show that the concentration of PM2.5 in Shihezi was 109.85 ± 58.76 μg·m-3 during the heating season, and the concentrations of heavy metals in PM2.5 were Fe(27.766 μg·m-3) > Zn(3.484 μg·m-3) > Pb(1.444 μg·m-3) > Cu(0.628 μg·m-3) > Ni(0.094 μg·m-3). Ni has a certain cancer risk, and Cu, Zn, and Pb have no non-carcinogenic risk. The increase of PM2.5 and Zn concentration significantly inhibited SOD activity and increased the levels of MDA, IL-6 and TNF-α. But for the two dosing groups with the same amount of Zn(PM2.5 high dose group and Zn medium dose group), PM2.5 damage to rats more severely. Therefore, PM2.5 and Zn element can induce damage to the lungs of rats. In this process, Zn plays an important role.
作者
任慧清
鲁建江
宁建英
苏向辉
童延斌
陈家灯
丁艳洲
REN Huiqing;LU Jianjiang;NING Jianying;SU Xianghui;TONG Yanbin;CHEN Jiadeng;DING Yanzhou(Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Production and Construction Corps/School of Chemistry and Chemical Engineering,Shihezi University,Shihezi,832003,China;The First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi,832002,China)
出处
《环境化学》
CAS
CSCD
北大核心
2020年第6期1716-1725,共10页
Environmental Chemistry
基金
国家自然科学基金(21667026)
新疆生产建设兵团社会科学基金(18YB13)资助
作者简介
通讯联系人:鲁建江,Tel: 0993-2055016,E-mail: lujianjiang_xj@ 163.com;通讯联系人:宁建英,Tel: 0993-2055016,E-mail: njy188@ sohu.com
