摘要
针对生物实验室污水处理难度高及其秋冬季节达标率低的问题,采用改进工艺的悬浮填料生物膜反应器(moving bed biofilm reactor,MBBR)进行连续处理,观察秋冬季节MBBR水质处理效果,利用高通量测序技术研究环境因子水温(T^w)、溶解氧(DO)、pH对生物膜细菌群落更替的影响以及主要微生物种群变化。结果表明,T^w由26℃下降到10℃期间,反应器COD、NH^+4-N去除率仍然分别保持在75%、80%左右,MBBR出水稳定在一级A标准。变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和浮霉菌门(Planctomycetes)是生物膜主要优势菌门,T^w的下降引起拟杆菌门的相对丰度显著升高。假单胞菌属(Pseudomonas)和黄杆菌属(Flavobacterium)在低T^w下成为优势菌属,动胶菌属(Zoogloea)相对丰度保持稳定。通过冗余分析(RDA)发现,DO与短波单胞菌属(Brevundimonas)、pH与脱氯单胞菌(Dechloromonas)、固氮弧菌属(Azoarcus)具有显著正相关性,T^w与假单胞菌属、黄杆菌属具有显著负相关性。MBBR结果揭示,细菌群落动态更替是MBBR出水水质仍然保持稳定的重要原因。
Biological laboratory wastewater is complicate and the effluent quality of its common treatment process is fluctuant during autumn and winter.An innovated moving bed biofilm reactor(MBBR)was used to continuously treat this wastewater,and its treatment effects were detected in autumn and winter.Highthroughput sequencing technology was used to explore the effects of environmental factors,such as water temperature(T^w),dissolved oxygen(DO),and pH,on alternation of bacterial communities,as well as the change of dominant genus.Results showed that when the system temperature drops from 26℃ to 10℃,the removal rates of COD and NH^+4-N were above 75%and 80%,respectively,and the effluent quality of MBBR met the first-level A standard.Proteobacteria,Bacteroidetes and Planctomycetes were the main dominant bacteria of biofilm,the relative abundance of Bacteroidetes increased significantly with the decline of temperature.Similarly,Pseudomonas and Flavobacterium finally became the dominant genus at low temperature,while the proportion of Zoogloea remained stable accordingly.Furthermore,Redundancy analysis(RDA)showed that DO was significantly positively correlated with Brevundimonas,pH was significantly positively correlated with Dechloromonas or Azoarcus,while T^w was negatively correlated with Pseudomonas or Flavobacterium.These results revealed that the dynamic alternation of bacterial community might be the key reasons for keeping stable effluent of MBBR.
作者
郝志鹏
张会宁
彭维
葛向阳
梁运祥
靳慧霞
陈正军
HAO Zhipeng;ZHANG Huining;PENG Wei;GE Xiangyang;LIANG Yunxiang;JIN Huixia;CHEN Zhengjun(State Key Laboratory of Agricultural Microbiology,College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Ningbo Institute of Technology,Zhejiang University,Ningbo 315000,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2019年第11期2601-2609,共9页
Chinese Journal of Environmental Engineering
基金
浙江省自然科学基金资助项目(2017C510006).
关键词
悬浮填料生物膜反应器
生物实验室污水
高通量测序
细菌群落
相对丰度
moving bed biofilm reactor(MBBR)
biological laboratory wastewater
high-throughput sequencing
bacterial community
relative abundance
作者简介
第一作者:郝志鹏(1992-),男,硕士研究生。研究方向:低浓度污水处理技术。E-mail:229266017@qq.com;通信作者:陈正军(1976-),男,博士,副教授。研究方向:微生物修复技术。E-mail:chenzhengjun@163.com.
